LOCUS       sequence-153903-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CBS_pCSdest
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgcc ttctgagacc ccccaggcag aagtggggcc cacaggctgc
      181 ccccaccgct cagggccaca ctcggcgaag gggagcctgg agaaggggtc cccagaggat
      241 aaggaagcca aggagcccct gtggatccgg cccgatgctc cgagcaggtg cacctggcag
      301 ctgggccggc ctgcctccga gtccccacat caccacactg ccccggcaaa atctccaaaa
      361 atcttgccag atattctgaa gaaaatcggg gacaccccta tggtcagaat caacaagatt
      421 gggaagaagt tcggcctgaa gtgtgagctc ttggccaagt gtgagttctt caacgcgggc
      481 gggagcgtga aggaccgcat cagcctgcgg atgattgagg atgctgagcg cgacgggacg
      541 ctgaagcccg gggacacgat tatcgagccg acatccggga acaccgggat cgggctggcc
      601 ctggctgcgg cagtgagggg ctatcgctgc atcatcgtga tgccagagaa gatgagctcc
      661 gagaaggtgg acgtgctgcg ggcactgggg gctgagattg tgaggacgcc caccaatgcc
      721 aggttcgact ccccggagtc acacgtgggg gtggcctggc ggctgaagaa cgaa
//
