LOCUS       sequence-154125-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW101ParsR-gfp
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(178..205)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(221..292)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actccagtga aaagttcttc tcctttacgc atctagtatt tctcctcttt aatctctagt
       61 attgcaggta gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat
      121 gtgtaagtgc agaggaaggt aataggtgtg aattttgagt tggctctagt atataaacgc
      181 agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac
      241 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
      301 ctctagtacc cggataaaac acatctgaaa attcatgtgt gtttagctaa atttttaact
      361 gcaaatgttc ttactgtccc cggaacagtt ttgtcgagcc aggttgcgga caatcgcctg
      421 aaccttttcc tgttcacatc gccaggcctc atcaataatt ttcgccgccc atgctggaat
      481 atgcggtgat aagcggtaat gaacccactt accttgcttg cggtccagca atagcccgct
      541 ttcacgcagc aatgccaggt ggcgggagat cttgggctgc gactggtcga gagcagtgca
      601 gagatcgcag acgcataacn ctcccagttc gctgagcagt aaaacgatgc ccagacgggt
      661 ttcatcagca agaattttga acaattggat gggtaacaga aatgacatat ggtactttcc
      721 tgtgtgaccc taggagctag cataa
//
