LOCUS       sequence-154138-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBW100ParsR carrying 30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW102ParsR-Amp32C
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(25..68)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        93..127
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      571..642
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      658..685
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
       61 atgatttctg gaattcgcgg ccgcttctag agtttacagc tagctcagtc cttggtatta
      121 tgctagctcc tagggtcaca caggaaagta ccatatgtca tttctgttac ccatccaatt
      181 gttcaaaatt cttgctgatg aaacccgtct gggcatcgtt ttactgctca gcgaactggg
      241 agagttatgc gtctgcgatc tctgcactgc tctcgaccag tcgcagccca agatctcccg
      301 ccacctggca ttgctgcgtg aaagcgggct attgctggac cgcaagcaag gtaagtgggt
      361 tcattaccgc ttatcaccgc atattccagc atgggcggcg aaaattattg atgaggcctg
      421 gcgatgtgaa caggaaaagg ttcaggcgat tgtccgcaac ctggctcgac aaaactgttc
      481 cggggacagt aagaacattt gcagttaaaa atttagctaa acacacatga attttcagat
      541 gtgttttatc cgggtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      601 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      661 tcaccttcgg gtgggccttt ctgcgtttat atactagagc caactcaaaa ttcacaccta
      721 ttaccttcct ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc
      781 gaagagagac actacctgca atactagagt cacacaggaa agtactagat gagtacaggc
      841 atcgataagg acgtccgaga gtgttgggg
//
