LOCUS       sequence-154187-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express and target GFP into the Golgi apparatus using 
            lentiviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  TITLE     pLV-mGFP Golgi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Golgi eGFP
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(117..830,831..833,834..836))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(162..183)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     511..530
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     770..791
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      833..842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
       61 attgtcgata tctgtacagg gccctgatca tccggagtcg accctgcagg tctagattac
      121 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      181 atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct caggtagtgg
      241 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      301 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      361 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      421 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      481 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      541 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      601 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
      661 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      721 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      781 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
      841 gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca gcggtgtgga gactccgacc
      901 agttggggca ggcggctcag gtcgcggcca gccaggtagt aaacgagggt gacgccaagg
      961 tgcagagcgc agacggccac gagcaggcgg caggcccgct gtagggacgc gcctggcatc
     1021 gcggcgctgc cgctcaggag cggctccc
//
