LOCUS       sequence-154249-        4280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a URA selection marker, 
            originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-g
FEATURES             Location/Qualifiers
     source          1..4280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(199..262)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        289..307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      1085..1272
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1140..1164)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1347..1690
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2509..2706
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2730..2763
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2808..2826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2859..3447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2939..2958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3618..4280,1..129,130..198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4241..4260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaagt atcgttcgaa ttaattaaaa ttaaccctca
      301 ctaaaggggc atgcgcggcc gctctagaac tatccaaggg tgaagagcta tttactgggg
      361 ttgtacccat tttggtagaa ctggacggag atgtaaacgg acataaattc tctgttagag
      421 gtgagggcga aggcgatgcc accaatggta aattgactct gaagtttata tgcactacgg
      481 gtaaattacc tgttccttgg ccaaccctag taacaacttt gacatatggt gttcaatgtt
      541 tctcaagata cccagaccat atgaaaaggc atgatttctt taaaagtgct atgccagaag
      601 gctacgtgca agagagaact atctccttta aggatgacgg tacgtataaa acacgagcag
      661 aagtgaaatt cgaaggggat acactagtta atcgcatcga attaaagggt atagacttta
      721 aggaagatgg taatattctc ggccataaac ttgagtataa tttcaactcg cataatgtgt
      781 acattacagc tgacaaacaa aagaacggaa ttaaagcgaa ttttaaaatc aggcacaacg
      841 tcgaagatgg gtctgttcaa cttgccgatc attatcagca aaacacccct attggtgatg
      901 gtccagtctt gttacccgat aatcactact taagcacaca gtctagattg tcaaaagatc
      961 cgaatgaaaa gcgtgatcac atggttttat tggaatttgt caccgctgca ggaataactc
     1021 acggaatgga cgagctttat aagatgctag cattttaact cgagggcgcg ccacttctaa
     1081 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     1141 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     1201 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
     1261 cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct
     1321 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
     1381 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
     1441 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
     1501 acggctcctc gcctcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
     1561 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
     1621 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
     1681 ataaacaacc atgacagtca acactaagac ctatagtgag agagcagaaa ctcatgcctc
     1741 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
     1801 aattgacgtt gataccacta aggagttcct tgaattaatt gataaattag gtccttatgt
     1861 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
     1921 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     1981 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     2041 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     2101 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     2161 atcatcagtg ggatcattag catacggaga atattctcaa aaaactgttg aaattgctaa
     2221 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     2281 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     2341 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     2401 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     2461 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     2521 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     2581 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     2641 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     2701 atactgctgt cgattcgata ctaacgccga taacttcgta taatgtatgc tatacgaagt
     2761 tattaatacg actcactata cgaattggag ctccaattcg gacgtcccct atagtgagtc
     2821 gtattattaa ttaattcgaa ggccgcgttg ctggcgtttt tccataggct cggcccccct
     2881 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2941 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3001 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3061 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3121 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3181 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3241 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3301 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3361 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3421 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3481 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3541 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3601 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3661 ctatttcgtt catccatagt tgcctgactg cccgtcgtgt agataactac gatacgggag
     3721 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3781 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3841 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3901 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3961 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4021 atgttgtgaa aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4081 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4141 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4201 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4261 agaactttaa aagtgctcat
//
