LOCUS       sequence-154373-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE148
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctcg gtctctgcaa gaattcaagc
      181 ttggagaatg gttataatat atagctttct atgaatgtat ggtgagaaaa tgtctgttca
      241 ctgattttga gtttggaata aaagcatttg cgtttggttt atcattgcgt ttatacaagg
      301 acagagatcc actgagctgg aatagcttaa aaccattatc agaacaaaat aaaccatttt
      361 ttgttaagaa tcagagcata gtaaacaaca gaaacaacct aagagaggta acttgtccaa
      421 gaagatagct aattatatct attttataaa agttatcata gtttgtaagt cacaaaagat
      481 gcaaataaca gagaaactag gagacttgag aatatacatt cttgtatatt tgtattcgag
      541 attgtgaaaa tttgaccata agtttaaatt cttaaaaaga tatatctgat ctagatgatg
      601 gttatagact gtaattttac cacatgttta atgatggata gtgacacaca tgacacatcg
      661 acaacactat agcatcttat ttagattaca acatgaaatt tttctgtaat acatgtcttt
      721 gtacataatt taaaagtaat tcctaagaaa tatatttata caaggagttt aaagaaaaca
      781 tagcataaag ttcaatgagt agtaaaaacc atatacagta tatagcataa agttcaatga
      841 gtttattaca aaagcattgg ttcactttct gtaacacgac gttaaacctt cgtctccaat
      901 aggagcgcta ctgattcaac atgccaatat atactaaata cgtttctaca gtcaaatgct
      961 ttaacgtttc atgattaagt gactatttac cgtcaatcct ttcccattcc tcccactaat
     1021 ccaacttttt aattactctt aaatcaccac taagcttcga atccatccaa aaccacaata
//
