LOCUS       sequence-154382-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE4
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             105..656
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      676..928
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
       61 ttagagtctc atattcactc tcaactcgat cgaggggatc taccatgagc ccagaacgac
      121 gcccggccga catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca
      181 accactacat cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt
      241 ggacggacga cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg
      301 gcgaggtcgc cggcatcgcc tacgcgggtc cctggaaggc acgcaacgcc tacgactgga
      361 cggccgagtc gaccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc
      421 tctacaccca cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca
      481 tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg
      541 gcatgctgcg ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc
      601 tggacttcag cctgccggtg ccgccccgtc cggtcctgcc cgtcaccgaa atctgatgac
      661 ccctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
      721 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
      781 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
      841 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
      901 cgcgcggtgt catctatgtt actagatcgg aattccgcaa tgtgttatta agttgtctaa
      961 gcgtcaattt gtttacacca caata
//
