LOCUS       sequence-154387-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE4
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ggtctctgca agaattcaag
      181 cttggagatt acggatatga atataggcat atccgtatcc gaattatccg tttgacagct
      241 agcaacgatt gtacaattgc ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa
      301 catcagcgtt aacaaacggc cccgttacgg cccaaacggt catatagagt aacggcgtta
      361 agcgttgaaa gactcctatc gaaatacgta accgcaaacg tgtcatagtc agatcccctc
      421 ttccttcacc gcctcaaaca caaaaataat cttctacagc ctatatatac aaccccccct
      481 tctatctctc ctttctcaca attcatcatc tttctttctc tacccccaat tttaagaaat
      541 cctctcttct cctcttcatt ttcaaggtaa atctctctct ctctctctct ctctgttatt
      601 ccttgtttta attaggtatg tattattgct agtttgttaa tctgcttatc ttatgtatgc
      661 cttatgtgaa tatctttatc ttgttcatct catccgttta gaagctataa atttgttgat
      721 ttgactgtgt atctacacgt ggttatgttt atatctaatc agatatgaat ttcttcatat
      781 tgttgcgttt gtgtgtacca atccgaaatc gttgattttt ttcatttaat cgtgtagcta
      841 attgtacgta tacatatgga tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat
      901 acagatctga aaacatcact tctctcatct gattgtgttg ttacatacat agatatagat
      961 ctgttatatc attttt
//
