LOCUS       sequence-154397-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE169
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    99..154
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
       61 ttagagtctc atattcactc tcatactgta tttttacaac aattaccaac aacaacaaac
      121 aacaaacaac attacaatta ctatttacaa ttacaatgaa aaagcctgaa ctcaccgcga
      181 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
      241 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
      301 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
      361 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
      421 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
      481 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
      541 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
      601 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
      661 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
      721 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
      781 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
      841 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
      901 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
      961 ttggtcttga ccaact
//
