LOCUS       sequence-154459-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter1 with BCD21-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Linshiz G, Stawski N, Goyal G, Bi C, Poust S, Sharma M, Mutalik V, 
            Keasling JD, Hillson NJ
  TITLE     PR-PR: cross-platform laboratory automation system.
  JOURNAL   ACS Synth Biol. 2014 Aug 15;3(8):515-24. doi: 10.1021/sb4001728. 
            Epub 2014 Jan 14.
  PUBMED    25126893
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001728";
            journalName: "ACS Synth Biol"; date: "2014-08-15- 15"; volume: "3"; 
            issue: "8"; pages: "515-24"
COMMENT     Sequence Label: pProm1_BCD21-GFP
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(5..718,719..721))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(8..721)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(48..68)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     663..691
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atccttattt gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa
       61 gaaggaccat gtggtcacgc ttttcgttgg gatctttcga aaggacagat tgtgtcgaca
      121 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      181 ggtctgctag ttgaacggaa ccatcttcaa cgttgtggcg aattttgaag ttagctttga
      241 ttccattctt ttgtttgtct gccgtgatgt atacattgtg tgagttaaag ttgtactcga
      301 gtttgtgtcc aagaatgttt ccatcttctt taaaatcaat accctttaac tcgatacgat
      361 taacaagggt atcaccttca aacttgactt cagcacgcgt cttgtaggtc ccgtcatctt
      421 tgaaagatat agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      481 gccgtttcat gtgatccgga taacgggaaa agcattgaac accataggtc agagtagtga
      541 caagtgttgg ccacggaaca ggtagttttc cagtagtgca aataaattta agggtgagtt
      601 ttccgtttgt agcatcacct tcaccctctc cacggacaga aaatttgtgc ccattaacat
      661 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttgctca
      721 ttagaaacca tccctcgcat gattaagatg tttcagtacg aaaattgctt tcattgttga
      781 tctccttttt aagtgaactt gggccctcca cacattatac ctataggtta gactttaagt
      841 caatactctt tttgttatgc agcaacgact catagaaagc acgcagctga tagggtcgac
      901 gtggtgtttg ctgtgcgttg gacgtgtgga ttgggatgaa aataccccca taaaaaaagg
      961 cgccgaagcg cc
//
