LOCUS       sequence-154501-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express wild type AlkB protein in E. coli BL21(DE3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Zheng G, Qin Y, Clark WC, Dai Q, Yi C, He C, Lambowitz AM, Pan T
  TITLE     Efficient and quantitative high-throughput tRNA sequencing.
  JOURNAL   Nat Methods. 2015 Sep;12(9):835-7. doi: 10.1038/nmeth.3478. Epub 
            2015 Jul 27.
  PUBMED    26214130
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3478"; journalName: "Nat Methods"; date: "2015-09"; 
            volume: "12"; issue: "9"; pages: "835-7"
COMMENT     Sequence Label: pET30a-AlkB
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgcaagag ccactggcgg ctggagcggt
      121 aattttacgg cgttttgctt ttaacgctgc ggagcaacta atccgcgata ttaatgacgt
      181 tgccagccag tcgccgtttc gccagatggt cacccccggg ggatatacca tgtcggtggc
      241 gatgaccaac tgtgggcatc tgggctggac gacccatcgg caaggttatc tctattcgcc
      301 cattgatccg caaacaaata aaccgtggcc cgccatgcca cagagttttc ataatttatg
      361 tcaacgtgcg gctacggcgg cgggctatcc agatttccag ccagatgctt gtcttatcaa
      421 ccgctacgct cctggcgcga aactgtcgct gcatcaggat aaagacgaac cggatctgcg
      481 cgcgccaatt gtttctgttt ctctgggctt acccgcgatt tttcaatttg gcggcctgaa
      541 acgtaatgat ccgctcaaac gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg
      601 tgaatcgcgg ctgttttatc acggtattca accgttgaaa gcggggtttc atccactcac
      661 catcgactgc cgctacaacc tgacattccg tcaggcaggt aaa
//
