LOCUS       sequence-154518-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SYNJ1-170_pCSdest
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gctccgcggc cgcccccttc accatggcgt tcagtaaagg atttcgaatc
      181 taccacaaat tggatccccc acctttcagc ctcatagtgg aaactaggca taaggaagaa
      241 tgtctcatgt tcgagtctgg ggctgtcgct gtgctctcat ctgcagaaaa agaggcaatc
      301 aagggtacat actccaaagt actggatgca tatggactct taggtgttct gcggttaaat
      361 cttggtgata ctatgttaca ttatctggtc ctagtcactg gatgtatgtc tgttggaaaa
      421 attcaagaat ctgaagtttt ccgagttact tccactgagt ttatatcact gcgaatcgat
      481 tcttcagatg aggatcgcat ttcagaagtg cggaaagttt tgaattcagg aaacttttat
      541 tttgcatggt ctgcatctgg catcagttta gatttgagtc ttaatgcgca tcgtagcatg
      601 caagaacaga caactgataa tagatttttc tggaatcagt ctttgcattt gcatctcaaa
      661 cactatggcg tgaattgtga tgactggtta ttacgtctta tgtgtggagg agtagaaatc
      721 agaacaattt atgctgctca taaacaggcg aaggcttgcc tcatttcaag attaagctgt
      781 gaacgagctg ggaccaggtt taatgtccgg g
//
