LOCUS       sequence-154526-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ZBTB21_pCSdest
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gctggtaccg gtccggaatt cccgggatat cgtcgaccca cgcgtccgtt
      181 cagataaagt gcaggatgga gggactgctg cattacatca acccagcaca tgctatctct
      241 ctgctcagtg ccctcaatga ggagcgcctc aagggacagc tgtgtgacgt gctcctgatt
      301 gttggggacc agaaattccg agctcataag aacgtcttgg ctgccagcag tgagtacttc
      361 cagagtttat tcacgaataa ggagaacgag gcacagactg tctttcagct ggacttctgt
      421 gagcctgacg cttttgacaa cgttctgaac tacatttact cttcttccct ttttgtggag
      481 aagggcagcc ttgctgctgt gcaggagctg gggtatagcc ttggtatctc cttcctgacc
      541 aacatcgttg ccaaagcccc tcaggctcct tttccagcct gtcccaacag gaaaagagtg
      601 ccagtggaag atgatgaaac cagctctcaa aagcgaagtg tcattgtgtg tcagggcaga
      661 agtgaagtgc cagggaaagc cagtggtcca gccatgcagg acctcagcca tactgcccgg
      721 gcctccccca gtgcttcagt caagaccacc atcagtaagc cacatgtggc caagccgcca
      781 gagcagcttc atagtctgcc cttaactgaa a
//
