LOCUS       sequence-154561-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_I13453. Has near identical expression as
            36INS1-pBAD_AB [E:36INS2-pBAD:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS2-pBAD_EB
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..90
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     178..197
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    286..306
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(312..329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(563..582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagag actagtgggt ctcagcttta gcacgctttt
      121 ctgcgtatgg ttggtcagct tctaacattg attatttgca cggcgtcaca ctttgctatg
      181 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
      241 ctactgtttc tccataccgt ttttttgggc tagctactag agacctacta gtagcggccg
      301 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      361 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      481 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      841 ggta
//
