LOCUS       sequence-155497-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing GSK3B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GSK3B_WT
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             818..829
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgtcagggc ggcccagaac cacctccttt gcggagagct gcaagccggt gcagcagcct
       61 tcagcttttg gcagcatgaa agttagcaga gacaaggacg gcagcaaggt gacaacagtg
      121 gtggcaactc ctgggcaggg tccagacagg ccacaagaag tcagctatac agacactaaa
      181 gtgattggaa atggatcatt tggtgtggta tatcaagcca aactttgtga ttcaggagaa
      241 ctggtcgcca tcaagaaagt attgcaggac aagagattta agaatcgaga gctccagatc
      301 atgagaaagc tagatcactg taacatagtc cgattgcgtt atttcttcta ctccagtggt
      361 gagaagaaag atgaggtcta tcttaatctg gtgctggact atgttccgga aacagtatac
      421 agagttgcca gacactatag tcgagccaaa cagacgctcc ctgtgattta tgtcaagttg
      481 tatatgtatc agctgttccg aagtttagcc tatatccatt cctttggaat ctgccatcgg
      541 gatattaaac cgcagaacct cttgttggat cctgatactg ctgtattaaa actctgtgac
      601 tttggaagtg caaagcagct ggtccgagga gaacccaatg tttcgtatat ctgttctcgg
      661 tactataggg caccagagtt gatctttgga gccactgatt atacctctag tatagatgta
      721 tggtctgctg gctgtgtgtt ggctgagctg ttactaggac aaccaatatt tccaggggat
      781 agtggtgtgg atcagttggt agaaataatc aaggtcctgg gaactccaac aagggagcaa
      841 atcagagaaa tgaacccaaa ctacacagaa tttaaattcc ctcaaattaa ggcacatcct
      901 tggactaagg tcttccgacc ccgaactcca ccggaggcaa ttgcactgtg tagccgtctg
      961 ctggagtata caccaactgc ccgactaaca ccactggaag cttgtgcaca ttcatttttt
     1021 gatgaattac gggacccaaa tgtcaaacta ccaaatgggc gagacacacc tgcactcttc
     1081 aacttcacca ctcaagaact gtcaagtaat ccacctctgg ctaccatcct tattcctcct
     1141 catgctcgga ttcaagcagc tgcttcaacc cccacaaatg ccacagcagc gtcagatgct
     1201 aatactggag accgtggaca gaccaataat gctgcttctg catcagcttc caactccacc
//
