LOCUS       sequence-155872-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to construct AAV-based targeting vectors that carry an 
            IVS-2Aneo cassette for promoter-trapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Karnan S, Ota A, Konishi Y, Wahiduzzaman M, Hosokawa Y, Konishi H
  TITLE     Improved methods of AAV-mediated gene targeting for human cell lines
            using ribosome-skipping 2A peptide.
  JOURNAL   Nucleic Acids Res. 2015 Dec 10. pii: gkv1338.
  PUBMED    26657635
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Dec 10. pii: gkv1338."
COMMENT     Sequence Label: pAAV-2Aneo v2
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          57..286
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     269..288
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             339..392
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(450..469)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ttatacgaag ttatggaatt tacccaattc gctgtctgcg agggccagct gttggggtga
       61 gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga
      121 ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg
      181 gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc
      241 atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag
      301 gtccaactgc attccggatg tacagtccgg actgtacaga gggcagagga agtcttctaa
      361 catgcggtga cgtggaggag aatcccggcc caaccatggc tgaacaagat ggattgcacg
      421 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      481 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      541 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      601 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      661 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      721 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      781 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
      841 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
      901 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
      961 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg ctttt
//
