LOCUS       sequence-155905-        9365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  No anti-scaffold control with 3xFLAG tags for Western blots. 
            Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH016
FEATURES             Location/Qualifiers
     source          1..9365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(132..1010)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1037..1321
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1244..1263
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2218..2289
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2305..2332
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2482..3195
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3196..3261
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3281..3310
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3326..3343
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3399..3416)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3569..3615
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3659..3679)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3659..3677)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3698..3714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3698..3714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3711..3733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3722..3738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3746..3752,3753..3770,3771..3776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3791..3812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4217..5011)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4328..4347)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4938..4957
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      6491..7260
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             7261..7923
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     8615..8637
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8630..8646
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8656..8675
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8656..8674
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      8715..8801
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8896..8982
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9077..9163
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9258..9344
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat
       61 tggggatcgg aagctgaatt cactagtcta tgctactcca tcgagccgtc aattgtctga
      121 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
      181 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
      241 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
      301 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
      361 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
      421 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
      481 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
      541 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
      601 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
      661 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
      721 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
      781 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
      841 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
      901 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
      961 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
     1021 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
     1081 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
     1141 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
     1201 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
     1261 tccataagat tagcggatct tacctgacgc tttttatcgc aactctctac tgtttctcca
     1321 tacccgctag cggtacctca cgcgatacag atctatgaaa ctgttgattg tcgaagatga
     1381 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     1441 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg atctgataat
     1501 cctcgatatt atgctgccgg acgtgaacgg ctgggatatc gtgcgcatgt tacgctccgc
     1561 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     1621 ggggctggag ttgggggcag atgactacct ggtgaagcca ttcgcttttg ctgaactgct
     1681 ggcgcgggtg cgcacattac tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     1741 ggttgccgat ttgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     1801 cactttgacc agtaaagagt ttactctgct ggagttcttc cttcgccatc agggcgaagt
     1861 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aattttgaca gcgataccaa
     1921 tgctattgat gtggcggtga agcggctgcg cggcaaaatc gacaacgact ttgagccgaa
     1981 gctaattcag accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagggatc
     2041 tgggtccggc agcggtggat ctctggaaat ccgcgcagcg tttcttgaaa aagaaaacac
     2101 cgcactgcgt acccgcgcgg ccgaactccg taaacgtgtg ggccggtgcc gcaacattgt
     2161 atccaaatac gaaacccgat acggcccgct ttaaggatcc tgactcgagc caggcatcaa
     2221 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2281 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatacc
     2341 gcggcctagg attggcaaaa tgacaatttt gtcatttttc tgtcaccgga aaatcagagc
     2401 ctggcgagta aagttggcgg cataaaatca ccagaaatta tgagctcata cattcgtaag
     2461 aaacgggcgc aggaggatta gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct
     2521 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt
     2581 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg
     2641 ccggttcctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt
     2701 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg
     2761 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa
     2821 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac
     2881 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc
     2941 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat
     3001 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt
     3061 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag
     3121 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg
     3181 gatgaactgt acaaagacta caaggaccat gacggtgatt acaaggatca tgacatcgac
     3241 tacaaggatg acgatgacaa gtagtgagaa gcttgggccc gaacaaaaac tcatctcaga
     3301 agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt aaacggtctc
     3361 cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
     3421 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     3481 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     3541 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     3601 ggcctttcgt tttatctgtt gtttgtcggt gaactaattt ctagaccagc ttttgttccc
     3661 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     3721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3901 gtttgcgtat tgggcgcatg cataaaaact gttgtaattc attaagcatt ctgccgacat
     3961 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     4021 cttgcgtata atatttgccc atgggggtgg gcgaagaact ccagcatgag atccccgcgc
     4081 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     4141 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     4201 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     4261 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     4321 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     4381 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4441 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     4501 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     4561 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     4621 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     4681 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     4741 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     4801 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     4861 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4921 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4981 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     5041 cagattttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     5101 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     5161 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     5221 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atcccaggtg
     5281 gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg
     5341 ctggacttcc cgctgttccg tcagcagctt ttcgcccacg gccttgatga tcgcggcggc
     5401 cttggcctgc atatcccgat tcaacggccc cagggcgtcc agaacgggct tcaggcgctc
     5461 ccgaaggtct cgggccgtct cttgggcttg atcggccttc ttgcgcatct cacgcgctcc
     5521 tgcggcggcc tgtagggcag gctcataccc ctgccgaacc gcttttgtca gccggtcggc
     5581 cacggcttcc ggcgtctcaa cgcgctttga gattcccagc ttttcggcca atccctgcgg
     5641 tgcataggcg cgtggctcga ccgcttgcgg gctgatggtg acgtggccca ctggtggccg
     5701 ctccagggcc tcgtagaacg cctgaatgcg cgtgtgacgt gccttgctgc cctcgatgcc
     5761 ccgttgcagc cctagatcgg ccacagcggc cgcaaacgtg gtctggtcgc gggtcatctg
     5821 cgctttgttg ccgatgaact ccttggccga cagcctgccg tcctgcgtca gcggcaccac
     5881 gaacgcggtc atgtgcgggc tggtttcgtc acggtggatg ctggccgtca cgatgcgatc
     5941 cgccccgtac ttgtccgcca gccacttgtg cgccttctcg aagaacgccg cctgctgttc
     6001 ttggctggcc gacttccacc attccgggct ggccgtcatg acgtactcga ccgccaacac
     6061 agcgtccttg cgccgcttct ctggcagcaa ctcgcgcagt cggcccatcg cttcatcggt
     6121 gctgctggcc gcccagtgct cgttctctgg cgtcctgctg gcgtcagcgt tgggcgtctc
     6181 gcgctcgcgg taggcgtgct tgagactggc cgccacgttg cccattttcg ccagcttctt
     6241 gcatcgcatg atcgcgtatg ccgccatgcc tgcccctccc ttttggtgtc caaccggctc
     6301 gacgggggca gcgcaaggcg gtgcctccgg cgggccactc aatgcttgag tatactcact
     6361 agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta cgggcttgct
     6421 ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg
     6481 atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga
     6541 caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct
     6601 acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat
     6661 caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg
     6721 ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg
     6781 gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc
     6841 cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc
     6901 accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt
     6961 gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg
     7021 caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa
     7081 ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag
     7141 aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa
     7201 gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc
     7261 atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg
     7321 caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg
     7381 gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc
     7441 cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag
     7501 gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg
     7561 gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg
     7621 gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat
     7681 ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc
     7741 ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg
     7801 accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg
     7861 tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg
     7921 tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg gctgtagccg
     7981 cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     8041 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt ttttatcagg
     8101 ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga
     8161 gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
     8221 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
     8281 gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     8341 agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     8401 ctcatcgcag tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8461 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
     8521 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8581 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     8641 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctggg gccgcaattc
     8701 ccaattccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     8761 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     8821 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     8881 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     8941 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     9001 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     9061 aattaattcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     9121 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     9181 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     9241 gaattaattc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     9301 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     9361 tgaac
//
