LOCUS       sequence-155938-        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-tagged ZnT1-WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Zogzas CE, Aschner M, Mukhopadhyay S
  TITLE     Structural Elements in the Transmembrane and Cytoplasmic Domains of 
            the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux
            Activity.
  JOURNAL   J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 
            10.1074/jbc.M116.726935. Epub 2016 Jun 15.
  PUBMED    27307044
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.726935"; journalName: "J Biol Chem"; date: 
            "2016-07-29- 29"; volume: "291"; issue: "31"; pages: "15940-57"
COMMENT     Sequence Label: FLAG-ZnT1-WT
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     gap             1073..1084
                     /estimated_length=12
     gap             1100..1119
                     /estimated_length=20
     gap             1139..1150
                     /estimated_length=12
     gap             1154..1176
                     /estimated_length=23
     gap             1189..1206
                     /estimated_length=18
ORIGIN
        1 gcnganctcg tttagtganc cgtcagaatt aaccatggac tacaaagacc atgacggtga
       61 ttataaagat catgacatcg attacaagga tgacgatgac aagcttgcgg ccgcgatggg
      121 gtgttggggt cggaaccggg gccggctgct gtgcatgctg gcgctgacct tcatgttcat
      181 ggtgctggag gtggtggtga gccgggtgac ctcgtcgctg gcgatgctct ccgactcctt
      241 ccacatgctg tcggacgtgc tggcgctggt ggtggcgctg gtggccgagc gcttcgcccg
      301 gcggacccac gccacccaga agaacacgtt cggctggatc cgagccgagg taatgggggc
      361 tctggtgaac gccatcttcc tgactggcct ctgtttcgcc atcctgctgg aggccatcga
      421 gcgcttcatc gagccgcacg agatgcagca gccgctggtg gtccttgggg tcggcgtggc
      481 cgggctgctg gtcaacgtgc tggggctctg cctcttccac catcacagcg gcttcagcca
      541 ggactccggc cacggccact cgcacggggg tcacggccac ggccacggcc tccccaaggg
      601 gcctcgcgtt aagagcaccc gccccgggag cagcgacatc aacgtggccc cgggcgagca
      661 gggtcccgac caggaggaga ccaacaccct ggtggccaat accagcaact ccaacgggct
      721 gaaattggac cccgcagacc cagaaaaccc cagaagtggt gatacagtgn aagtacaagt
      781 gaatggaaat cttgtcanan aacctgacca tatggaactg gaagaagana gggctggaca
      841 acttaacatg cgtggagttt ttctgcatgt ccttggagat gccttgggtt cagtgattgt
      901 antantaaat gccttantct tttacttttc ntggnaaggt tgtnctgann gggatttttg
      961 tgtgaatcca tnnttccctg acccctgcna agcattnnan aantnannaa nagtnnnnnt
     1021 gcatcanttn nnganntngn cnnnnctngg nnctannntt nnancnnnnn ttnnnnnnnn
     1081 nnnntnngnn nnnnnnnttn nnnnnnnnnn nnnnnnnnng nannngnnnn tnnnnnntnn
     1141 nnnnnnnnnn tncnnnnnnn nnnnnnnnnn nnnnnnantn nnnnntnann nnnnnnnnnn
     1201 nnnnnnannn nnnc
//
