LOCUS       sequence-155963-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_R0040. [F:36INS-pTet:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-pTet_FB
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..51
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    57..77
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     protein_bind    99..117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    197..218
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      221..264
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(268..286)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        354..458
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(677..696)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc
       61 agcggccgct actagtaggt ctctagtagt gctcagtatc tctatcactg atagggatgt
      121 caatctctat cactgatagg gaacaacagt gaggtttctt tactatgtat tctctccgag
      181 cgtgagaccc actagtctct agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag
      241 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
      301 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      361 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      421 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      481 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      541 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      601 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      661 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggc
//
