LOCUS       sequence-155975-        9965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector, Zeocin-2A-sHRPbNLG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1
FEATURES             Location/Qualifiers
     source          1..9965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1072..1375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1378..1653
                     /label=chicken beta-actin promoter
     intron          1654..2662
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2586..2608
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    2729..2753
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2897..3268
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      3533..3542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3986..4027
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4489..4498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6434..6463
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6550..6569)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6615..6670
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6669..6688)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   7291..7325
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7816..7832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7816..7832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7829..7851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7840..7856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7864..7870,7871..7888,7889..7894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7909..7930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8047..8064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8218..8806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8298..8317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8977..9768,9769..9837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9600..9619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9838..9942)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc gggggatcca ctagttatta
     1021 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1081 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1141 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1201 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1261 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     1321 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg
     1381 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1441 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
     1501 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
     1561 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     1621 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
     1681 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     1741 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     1801 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
     1861 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     1921 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1981 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     2041 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2101 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     2161 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     2221 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     2281 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2341 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     2401 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     2461 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2521 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     2581 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2641 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2701 ttttggcaaa gaattcctcg aggatatcac aagtttgtac aaaaaagcag gctccgaatt
     2761 cgcccttcgt aactataacg gtcctaaggt agcgaatagt gaaccgtcag atccgctagc
     2821 gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     2881 ccgggatcga tccaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     2941 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     3001 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     3061 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     3121 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     3181 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     3241 cgtgcacttc gtggccgagg agcaggacac tagtagtact aacttcgacc tgctgaagct
     3301 ggccggcgac gtggagagca atcctggacc taattttgat ctcctcaaac tcgctggaga
     3361 tgtcgaatcc aatcctggac ctaactttga cttgttgaag ttggcagggg acgttgagtc
     3421 taacccaggg ccagatatcg cggccgcgat gttcatgcct tcttcttttt cctacagctc
     3481 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ccgccaccat
     3541 ggcacttccc agatgcatgt ggccaaatta tgtttggaga gctatgatgg catgtgtggt
     3601 ccacagggga tccggtgccc cattgactct ctgcttgttg ggatgtttgc tacagacttt
     3661 tcacgtactc tctcaaaaga ccggtaactt gtctgcactg gttgactttg acctgcgcac
     3721 cccaaccatc ttcgacaata agtattacgt caatctggag gaacagaagg gcctgattca
     3781 gtccgaccag gagctgttca gttccccaaa tgctaccgac actattcctc ttgtgcgcag
     3841 cttcgctaat agcacacaaa cctttttcaa tgctttcgtg gaagctatgg atcggatggg
     3901 aaatataacg cccctgacgg gtacgcaggg acagataaga ctgaactgta gggtcgtgaa
     3961 cagcaactct ggctcgggct cgggcggcaa gcccatcccc aaccccctgc tgggcctgga
     4021 cagcacccaa ttgttggatg atgtagaccc attggttact actaactttg gcaagattag
     4081 gggaattaag aaagaactca ataatgaaat tttgggtcct gtcattcagt ttcttggggt
     4141 tccatatgcc gctccaccaa caggagaaca tcgtttccag cctccagaac caccatctcc
     4201 ctggtctgac atccggaacg ccactcagtt tgctcctgta tgtccccaga atatcattga
     4261 tggcagattg cctgaagtta tgcttcctgt gtggttcact aataacttgg atgtggtttc
     4321 atcatacgtc caagaccaga gtgaagactg tctatactta aacatctatg tcccaactga
     4381 agatggtccc cttacaaaga aacacacaga tgatttaggt gataatgacg gtgctgaaga
     4441 tgaagatatt cgggacagtg ggggtcccaa accagtgatg gtgtacatcc atggcggctc
     4501 ttacatggaa ggtactggaa atctgtatga tgggagtgtc ttggcaagct atggcaatgt
     4561 gatcgtcatc acagtcaact atcggcttgg ggtacttggc ttcttgagca caggggatca
     4621 ggctgccaaa ggaaactacg ggctccttga cctcatccag gccctaagat ggaccagcga
     4681 gaacattggg ttctttggtg gtgacccctt gcgaatcact gtgtttggat caggcgctgg
     4741 gggttcatgt gtcaacctgc tgactttatc ccattattct gaaggtaacc gttggagcaa
     4801 ttcaaccaaa ggactttttc aacgagcaat agctcagagt ggaacagccc tttccagctg
     4861 ggctgttagt ttccagcctg caaaatacgc tagaattctg gccacaaaag ttggctgcaa
     4921 tgtttcagat acagtagagt tagtagaatg cctgcagaag aagccttaca aagaacttgt
     4981 tgatcaagat gttcaaccag cccgatacca catagccttt ggacctgtga tcgatggtga
     5041 tgtaatacca gatgaccctc agatactgat ggaacaagga gagttcctca actatgatat
     5101 aatgttggga gttaaccaag gggaagggtt gaagtttgtc gaaaatatag tagatagtga
     5161 tgatggtgta tcagccagtg atttcgactt tgctgtttct aattttgttg ataatttata
     5221 tggatatcct gaaggcaaag atgttttgag agaaaccatt aaattcatgt atactgactg
     5281 ggctgatcgc cataatcctg aaactagaag gaagacattg ttggctttgt ttacggacca
     5341 tcaatgggta gcacctgctg tggccacagc agaccttcac tcgaactttg gctcacctac
     5401 atacttctat gccttttatc atcattgcca aacagatcaa gttccagctt gggctgatgc
     5461 agctcatggg gatgaggttc cctatgtgtt gggaatcccc atgattggcc ctacagagtt
     5521 atttccttgc aatttctcca agaatgatgt gatgttgagt gcagtagtaa tgacatactg
     5581 gacgaatttt gctaaaactg gtgacccaaa tcaaccagtt cctcaagaca caaaattcat
     5641 ccataccaaa cccaaccgct ttgaagaagt agcatggacc agatattccc agaaagacca
     5701 gctttatctc catattggat taaaaccgag agttaaagag cattacagag ccaataaggt
     5761 aaatctctgg ctggagctgg tacctcatct gcataatctc aatgacattt ctcagtatac
     5821 ctcgacaaca actaaagtgc catccacgga catcactctc agacctacaa ggaagaattc
     5881 cacaccagtc acatcagcct ttcccactgc caaacaggat gatcccaagc aacaaccaag
     5941 ccccttctcg gtggatcaga gggactactc cacagagcta agtgtcacta tcgcagtggg
     6001 ggcctctctg ctgtttctca acatcttggc ttttgcagcc ctgtactaca agaaggataa
     6061 gaggagacat gatgtccacc ggaggtgcag ccctcagcgc acgaccacca acgacctaac
     6121 ccatgctcca gaagaggaaa ttatgtctct ccaaatgaag cacactgact tggatcacga
     6181 gtgtgagtcc atccatccac atgaggtggt tcttcggacc gcctgtcccc cagattatac
     6241 tctagctatg aggaggtcac ctgatgatat tccactaatg acacctaaca ccatcacaat
     6301 gattcccaac actataccag ggattcagcc cttacataca ttcaacacat ttactggagg
     6361 acagaataat acactgcccc atccccaccc acacccccat tcacattcaa caaccagggt
     6421 atagggcgcg ccagagcaaa agctcatttc tgaagaggac ttatgaggcc gcactcctca
     6481 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     6541 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     6601 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     6661 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     6721 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     6781 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     6841 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     6901 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     6961 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagcttgata
     7021 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     7081 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     7141 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     7201 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     7261 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     7321 tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     7381 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     7441 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     7501 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     7561 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     7621 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     7681 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     7741 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     7801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7861 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7921 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7981 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8041 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8101 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8641 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9961 gccac
//
