LOCUS       sequence-156049-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the active catalytic domain (amino acid 
            residues 164-468) of YopH. Inteded for co-expression with human Tyr 
            kinase plasmids to enhance bacterial kinase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: YopH (residues 164-468)
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataga tcatgcgtga acgaccacac acttctggcc atcacggggc tggcgaagcc
      121 agagccaccg caccaagcac tgtttctcct tatggcccag aagcgcgcgc agaactcagc
      181 agccgcctca ccacattgcg caatacgctg gcgccagcaa cgaatgatcc gcgttactta
      241 caagcctgcg gcggtgaaaa gctaaaccga tttagagata ttcaatgctg tcggcaaacc
      301 gcagtacgcg ccgatcttaa tgccaattac atccaggtcg gtaacactcg taccatagcg
      361 tgccagtatc cgctacaatc tcaacttgaa agccatttcc gtatgctggc agaaaaccga
      421 acgccagtgt tggctgtttt agcgtccagt tctgagatag ccaatcaaag attcggtatg
      481 ccagattatt tccgccagag tggtacctat ggcagtatca ctgtagagtc taaaatgact
      541 cagcaagttg gtctcggtga cgggattatg gcagatatgt atactttaac gattcgtgaa
      601 gcgggtcaaa aaacaatctc tgttcctgtg gttcatgttg gcaattggcc cgatcagacc
      661 gcagtcagct ctgaagttac caaggcactc gcttcactgg tagatcaaac agcagaaaca
      721 aaacgcaata tgtatgaaag caaaggaagt tcagcggtag gagatgactc caaattacgg
      781 ccggtaatac attgccgtgc gggtgttggc cgtactgcgc aactgattgg cgca
//
