LOCUS       sequence-156090-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of VRK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: VRK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca accacctcac ttgaagcttt gcccacaggg acagtgctga
      181 cagacaagag tgggcgacag tggaagctga agtccttcca gaccagggac aaccagggca
      241 ttctctatga agctgcaccc acctccaccc tcacctgtga ctcaggacca cagaagcaaa
      301 agttctcact caaactggat gccaaggatg ggcgcttgtt caatgagcag aacttcttcc
      361 agcgggccgc caagcctctg caagtcaaca agtggaagaa gctgtactcg accccactgc
      421 tggccatccc tacctgcatg ggtttcggtg ttcaccagga caaatacagg ttcttggtgt
      481 tacccagcct ggggaggagc cttcagtcgg ccctggatgt cagcccaaag catgtgctgt
      541 cagagaggtc tgtgctgcag gtggcctgcc ggctgctgga tgccctggag ttcctccatg
      601 agaatgagta tgttcatgga aatgtgacag ctgaaaatat ctttgtggat ccagaggacc
      661 agagtcaggt gactttggca ggctatggct tcgccttccg ctattgccca agtggcaaac
      721 acgtggccta cgtggaaggc agcaggagcc ctcacgaggg ggaccttgag ttcattagca
      781 tggacctgca caagggatgc gggccctccc gccgcagcga cctccagagc ctgggctact
      841 gcatgctgaa gtggctctac gg
//
