LOCUS       sequence-156245-        9290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: Vinculin-venus
FEATURES             Location/Qualifiers
     source          1..9290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4124..4126,4127..4129,4130..4843)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4169..4190)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4430..4449)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4777..4798
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4881..4898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4887..5111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5157..5585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5244..5263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5454..5475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5594..5614)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5599..5928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5779..5914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5841..5860
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5995..6789
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6049..6068)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6659..6678
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6963..7084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7000..7019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7054..7073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7133..7149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7133..7149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7146..7168)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7157..7173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7181..7187,7188..7205,7206..7211))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7226..7247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7364..7381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7535..8123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7615..7634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8294..9085,9086..9154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8917..8936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9155..9259)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc accatcgaga gcatcttgga
      961 gcccgtggct cagcagatct cccacctggt catcatgcac gaggaggggg aggtagacgg
     1021 caaggccatc ccggacctca ccgcccccgt gtcggccgtg caggccgctg tcagcaacct
     1081 ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac cagatcttga aaagggatat
     1141 gccaccagca ttcatcaaag tagagaatgc ctgcaccaag ctcgttcgag cagcccagat
     1201 gctgcaagca gatccttatt cagtaccagc tcgtgactac ctaattgatg gatcaagagg
     1261 catcctttct ggaacatcag acttacttct gacatttgat gaagcggagg tccgtaaaat
     1321 catccgtgtc tgcaaaggaa tattggaata tctgactgtg gcagaagtag tagagactat
     1381 ggaggatttg gtgacatata caaagaatct agggccagga atgacaaaga tggcgaaaat
     1441 gatcgatgag agacaacagg aattaactca tcaggaacat agggttatgc tggtgaactc
     1501 catgaatact gtgaaggagc tattgcctgt acttatttca gctatgaaga tctttgtaac
     1561 cacaaaaaac actaaaagcc agggaataga agaggccttg aaaaatcgca atttcacagt
     1621 agagaaaatg agtgctgaga taaatgaaat aatccgtgta ttacaactca cttcctggga
     1681 tgaagatgcc tgggccagca aggacactga agccatgaaa agagctttag ccctaataga
     1741 ttcaaagatg aaccaggcaa aaggctggct gagagatcca aacgcacctc caggggatgc
     1801 tggtgagcaa gcaataaggc agatccttga tgaagctgga aaagcaggag aattgtgtgc
     1861 aggcaaagaa cgcagggaga ttctgggaac atgcaaaact ttgggccaaa tgactgatca
     1921 acttgctgat ctccgagcta gaggacaggg tgctacaccg atggcaatgc agaaggcaca
     1981 gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg gagaatgcag cccggaaatt
     2041 ggaggccatg acaaactcta agcaggctat tgcaaagaag attgatgctg ctcagaactg
     2101 gcttgcggat cccaacggcg gaagtgaagg agaagaacac attcgaggaa ttatgtctga
     2161 agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa agagatgata tccttcgctc
     2221 cttgggggaa atctctgctc tgacagctaa gctgtcagat ctgcgacgac atgggaaagg
     2281 cgactctcct gaggcccgtg cattggccaa gcaaatagct acatcacttc agaacttaca
     2341 gtccaaaaca aacagagctg tagcaaatac taggccagtt aaagctgctg tccatttgga
     2401 gggcaagatt gagcaagccc agaggtggat agataatcct acagttgatg atcggggagt
     2461 aggccaggca gcaattcggg gtttggttgc agaaggtcgt cgtctagcca atgtcatgat
     2521 gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt gtagaccagc tggctgctca
     2581 gcttgctgac cttgcagcaa gaggggaagg agagtctcct caggctaggg caattgctgc
     2641 tcagcttcag gactccctga aggatctcaa agcacggatg caagaagcaa tgacccagga
     2701 ggtgtctgat gttttcagtg acactacaac ccctattaag ttgttagcag tagcagccac
     2761 tgctccttct gatactccca atagagaaga ggtgtttgaa gaaagagcag caaattttga
     2821 aaaccatgct gctagactgg gagcaacagc agaaaaagca gctgcagttg gaactgctaa
     2881 taaaactact gtggaaggca ttcaggcaac agtcaaatct gcaagggagc ttacaccaca
     2941 ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga aatcaagctg cttatgagca
     3001 ttttgagaca atgaaaaacc aatggattga taatgtagaa aagatgacag ggctggtgga
     3061 tgaggccatc gataccaagt ctctgttgga tgcatcagaa gaggctatta agaaggatct
     3121 tgataaatgt aaagttgcaa tggccaatat gcaacctcag atgctggtag ctggagccac
     3181 cagcattgct agacgagcga accgcatcct gcttgtggca aaacgggagg ttgaaaattc
     3241 agaagaccct aaattcaggg aggctgttaa agcagcttct gatgagctga gcaaaaccat
     3301 atcaccgatg gtaatggatg ctaaagctgt agcaggaaat atctctgatc ctggtttgca
     3361 gaagagtttc ttggattctg gatacaggat tctgggagct gtggccaaag tcagagaagc
     3421 ctttcagcct caggagccag actttccccc tcctcctcct gacctagagc atctccatct
     3481 gactgatgag cttgctcctc caaaaccacc acttccagaa ggtgaggttc ccccacccag
     3541 accaccaccc cctgaagaaa aagatgagga gttcccagag cagaaagcag gagaagctat
     3601 taatcagccc atgatgatgg ctgctaggca gttgcatgac gaggctcgga aatggtctag
     3661 caagggtaac gacatcattg ctgctgctaa acgaatggcg ctgctcatgg ctgagatgtc
     3721 acgcctggtg cgaggaggca gcggaaacaa gcgtgccctc atccagtgtg caaaagatat
     3781 tgctaaggca tcggatgaag tcactcgatt ggccaaagag gtggccaagc aatgtactga
     3841 taaacgcatt agaacaaacc tcttacaggt ctgtgagcga atcccaacca tcagcacgca
     3901 gctcaaaatt ctttccacag tgaaagctac catgctgggc aggactaaca tcagcgatga
     3961 agaatcagaa caggcaactg agatgttggt tcataacgcc cagaacctca tgcagtctgt
     4021 gaaggaaact gtgagagaag ctgaagcagc atccattaag ataagaacag atgccggatt
     4081 cactctgcgc tgggtcagaa agaccccatg gtatcaggaa ttcatggtga gcaagggcga
     4141 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4201 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4261 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     4321 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4381 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4441 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4501 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4561 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     4621 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     4681 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4741 caaactgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4801 cgccgccggg atcactctcg gcatggacga gctgtacaag taaatcgatt ctagagggcc
     4861 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5101 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5221 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5281 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5341 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5461 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5521 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5581 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5641 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5701 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5761 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5821 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5881 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5941 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6001 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6061 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6121 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6181 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6241 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6301 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6361 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6421 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6481 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6541 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6601 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6661 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6721 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6781 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6841 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6901 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6961 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7081 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7141 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7201 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7261 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7321 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8041 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8101 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8161 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8221 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8281 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8341 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8401 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8461 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8521 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8581 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8641 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8701 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8761 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8821 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8881 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8941 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9001 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9061 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9121 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9181 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9241 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
