LOCUS       sequence-156335-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 
            expression. Contains NsiI cloning site for U6-sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Dow LE, Fisher J, O'Rourke KP, Muley A, Kastenhuber ER, Livshits G, 
            Tschaharganeh DF, Socci ND, Lowe SW
  TITLE     Inducible in vivo genome editing with CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 
            2015 Feb 18.
  PUBMED    25690852
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3155"; 
            journalName: "Nat Biotechnol"; date: "2015-04"; volume: "33"; issue:
            "4"; pages: "390-4"
COMMENT     Sequence Label: c3GIC9
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    75..122
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    join(155..220,221..258)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    427..561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    681..815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    857..875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    893..911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    929..947
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    965..983
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1001..1019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc gatcgagcag
       61 ctgaagctta tggagaagtt actattccga agttcctatt ctctagaaag tataggaact
      121 tcgatccccc tcgatccccc gggctgcaga tctgtagggc gcagtagtcc agggtttcct
      181 tgatgatgtc atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact
      241 cttcgcggtc tttccagtgg ggatcgacgg tatcgataag cttgcagatc tgcgactcta
      301 gaggatctgc gactctagag gatcataatc agccatacca catttgtaga ggttttactt
      361 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      421 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      541 gtatcttatc atgtctggat ctgcgactct agaggatcat aatcagccat accacatttg
      601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      661 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      781 ccaaactcat caatgtatct tatcatgtct ggatcgagtt taccatgcat gtgttcgatt
      841 ctagattcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
      901 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      961 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     1021 cgtatctac
//
