LOCUS       sequence-156340-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 
            expression. Contains NsiI cloning site for U6-sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Dow LE, Fisher J, O'Rourke KP, Muley A, Kastenhuber ER, Livshits G, 
            Tschaharganeh DF, Socci ND, Lowe SW
  TITLE     Inducible in vivo genome editing with CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 
            2015 Feb 18.
  PUBMED    25690852
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3155"; 
            journalName: "Nat Biotechnol"; date: "2015-04"; volume: "33"; issue:
            "4"; pages: "390-4"
COMMENT     Sequence Label: c3GIC9
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..129)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcgaaaaaga aagaacaatc aagggtcccc aaactcaccc tgaagttctc aacgctcgag
       61 cactgtgctg gcgaattctt actttttctt ttttgcctgg ccggcctttt tcgtggccgc
      121 cggccttttg tcgcctccca gctgagacag gtcgatccgt gtctcgtaca ggccggtgat
      181 gctctggtgg atcagggtgg cgtccagcac ctctttggtg ctggtgtacc tcttccggtc
      241 gatggtggtg tcaaagtact tgaaggcggc aggggctccc agattggtca gggtaaacag
      301 gtggatgata ttctcggcct gctctctgat gggcttatcc cggtgcttgt tgtaggcgga
      361 cagcactttg tccagattag cgtcggccag gatcactctc ttggagaact cgctgatctg
      421 ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca aacagctgtt tctgctcatt
      481 atcctcgggg gagcccttca gcttctcata gtggctggcc aggtacagga agttcacata
      541 tttggagggc agggccagtt cgtttccctt ctgcagttcg ccggcagagg ccagcattct
      601 cttccggccg ttttccagct cgaacaggga gtacttaggc agcttgatga tcaggtcctt
      661 tttcacttct ttgtagccct tggcttccag aaagtcgatg ggattcttct cgaagctgct
      721 tctttccatg atggtgatcc ccagcagctc tttcacactc ttcagtttct tggacttgcc
      781 cttttccact ttggccacca ccagcacaga ataggccacg gtggggctgt cgaagccgcc
      841 gtacttctta gggtcccagt ccttctttct ggcgatcagc ttatcgctgt tcctcttggg
      901 caggatagac tctttgctga agccgcctgt ctgcacctcg gtctttt
//
