LOCUS       sequence-156363-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A 
            variant) expression. Contains NsiI cloning site for U6-sgRNA 
            cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Dow LE, Fisher J, O'Rourke KP, Muley A, Kastenhuber ER, Livshits G, 
            Tschaharganeh DF, Socci ND, Lowe SW
  TITLE     Inducible in vivo genome editing with CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 
            2015 Feb 18.
  PUBMED    25690852
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3155"; 
            journalName: "Nat Biotechnol"; date: "2015-04"; volume: "33"; issue:
            "4"; pages: "390-4"
COMMENT     Sequence Label: c3GIC9n
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..111)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcgaaaaaga aagaacaatc aagggtcccc aaactcaccc tgaagttctc aacgctcgag
       61 cactgtgctg gcgaattctt agctggcctc cacctttctc ttcttcttgg ggctgtcgcc
      121 tcccagctga gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag
      181 ggtggcgtcc agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa
      241 gtacttgaag gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc
      301 ggcctgctct ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag
      361 attagcgtcg gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc
      421 caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc
      481 cttcagcttc tcatagtggc tggccaggta caggaagttc acatatttgg agggcagggc
      541 cagttcgttt cccttctgca gttcgccggc agaggccagc attctcttcc ggccgttttc
      601 cagctcgaac agggagtact taggcagctt gatgatcagg tcctttttca cttctttgta
      661 gcccttggct tccagaaagt cgatgggatt cttctcgaag ctgcttcttt ccatgatggt
      721 gatccccagc agctctttca cactcttcag tttcttggac ttgccctttt ccactttggc
      781 caccaccagc acagaatagg ccacggtggg gctgtcgaag ccgccgtact tcttagggtc
      841 ccagtccttc tttctggcga tcagcttatc gctgttcctc ttgggcagga tagactcttt
      901 gctgaagccg cctgtctgca cctcggtctt tttcacgata ttcacttggg gcatgctcag
      961 cactttccgc acggtggcaa a
//
