LOCUS       sequence-156380-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AKT1_p.V201I
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
       61 tggcaccatg agcgacgtgg ctattgtgaa ggagggttgg ctgcacaaac gaggggagta
      121 catcaagacc tggcggccac gctacttcct cctcaagaat gatggcacct tcattggcta
      181 caaggagcgg ccgcaggatg tggaccaacg tgaggctccc ctcaacaact tctctgtggc
      241 gcagtgccag ctgatgaaga cggagcggcc ccggcccaac accttcatca tccgctgcct
      301 gcagtggacc actgtcatcg aacgcacctt ccatgtggag actcctgagg agcgggagga
      361 gtggacaacc gccatccaga ctgtggctga cggcctcaag aagcaggagg aggagatgga
      421 cttccggtcg ggctcaccca gtgacaactc aggggctgaa gagatggagg tgtccctggc
      481 caagcccaag caccgcgtga ccatgaacga gtttgagtac ctgaagctgc tgggcaaggg
      541 cactttcggc aaggtgatcc tggtgaagga gaaggccaca ggccgctact acgccatgaa
      601 gatcctcaag aaggaagtca tcgtggccaa ggacgaggtg gcccacacac tcaccgagaa
      661 ccgcatcctg cagaactcca ggcacccctt cctcacagcc ctgaagtact ctttccagac
      721 ccacgaccgc ctctgctttg tcatggagta cgccaacggg ggcgagctgt tcttccacct
      781 gtcccgggag cgtgtgttct ccgaggaccg ggcccgcttc tatggcgctg agattgtgtc
      841 agccctggac tacctgcact cggagaagaa cgtggtgtac cgggacctca agctggagaa
      901 cctcatgctg gacaaggacg ggcacattaa gatcacagac ttcgggctgt gcaaggaggg
      961 gatcaaggac ggtgccacca tgaagacctt ttgcggcaca cctgagtacc tggcccccga
     1021 ggtgctggag
//
