LOCUS       sequence-156397-       12421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-hM4Di-mCherry
FEATURES             Location/Qualifiers
     source          1..12421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(955..974)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1451..1471
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1586..1603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1592..1816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1822..2201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2203..2480
                     /label=chicken beta-actin promoter
     intron          2482..3499
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3423..3445
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3507..3526
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3563..3572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5021..5030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5027..5737
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5172..5190)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5440..5459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5776..6364
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5829..5849)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6247..6258)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6396..6872
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6595..6614)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6982..7001)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7047..7102
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7101..7120)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7463..7479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7487..7503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7511..7517,7518..7535,7536..7541))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7556..7577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7651..8487
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8527..8546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8528..8546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8553..8570)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8553..8569)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8562..8584)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8707..9009
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8888..8907
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(9412..9431)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10026..10045
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10406..11197,11198..11266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11029..11048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11390..11978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11879..11898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12132..12149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12266..12287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12302..12307,12308..12325,12326..12332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12340..12356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12364..12380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12401..12419
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
      961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1801 gcggtgggct ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta
     1861 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1921 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1981 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2041 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2101 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2161 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     2221 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     2281 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg
     2341 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
     2401 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     2461 agcgaagcgc gcggcgggcg gggagtcgct gcgacgctgc cttcgccccg tgccccgctc
     2521 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     2581 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     2641 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     2701 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     2761 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     2821 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     2881 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     2941 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     3001 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     3061 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     3121 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     3181 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     3241 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     3301 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     3361 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     3421 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     3481 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     3541 tggcaaagaa ttcgtcgacg ccgccaccat ggccaacttc acacctgtca atggcagctc
     3601 gggcaatcag tccgtgcgcc tggtcacgtc atcatcccac aatcgctatg agacggtgga
     3661 aatggtcttc attgccacag tgacaggctc cctgagcctg gtgactgtcg tgggcaacat
     3721 cctggtgatg ctgtccatca aggtcaacag gcagctgcag acagtcaaca actacttcct
     3781 cttcagcctg gcgtgtgctg atctcatcat aggcgccttc tccatgaacc tctacaccgt
     3841 gtacatcatc aagggctact ggcccctggg cgccgtggtc tgcgacctgt ggctggccct
     3901 ggactgcgtg gtgagcaacg cctccgtcat gaaccttctc atcatcagct ttgaccgcta
     3961 cttctgcgtc accaagcctc tcacctaccc tgcccggcgc accaccaaga tggcaggcct
     4021 catgattgct gctgcctggg tactgtcctt cgtgctctgg gcgcctgcca tcttgttctg
     4081 gcagtttgtg gtgggtaagc ggacggtgcc cgacaaccag tgcttcatcc agttcctgtc
     4141 caacccagca gtgacctttg gcacagccat tgctggcttc tacctgcctg tggtcatcat
     4201 gacggtgctg tacatccaca tctccctggc cagtcgcagc cgagtccaca agcaccggcc
     4261 cgagggcccg aaggagaaga aagccaagac gctggccttc ctcaagagcc cactaatgaa
     4321 gcagagcgtc aagaagcccc cgcccgggga ggccgcccgg gaggagctgc gcaatggcaa
     4381 gctggaggag gcccccccgc cagcgctgcc accgccaccg cgccccgtgg ctgataagga
     4441 cacttccaat gagtccagct caggcagtgc cacccagaac accaaggaac gcccagccac
     4501 agagctgtcc accacagagg ccaccacgcc cgccatgccc gcccctcccc tgcagccgcg
     4561 ggccctcaac ccagcctcca gatggtccaa gatccagatt gtgacgaagc agacaggcaa
     4621 tgagtgtgtg acagccattg agattgtgcc tgccacgccg gctggcatgc gccctgcggc
     4681 caacgtggcc cgcaagttcg ccagcatcgc tcgcaaccag gtgcgcaaga agcggcagat
     4741 ggcggcccgg gagcgcaaag tgacacgaac gatctttgcc attctgctgg ccttcatcct
     4801 cacctggacg ccctacaacg tcatggtcct ggtgaacacc ttctgccaga gctgcatccc
     4861 tgacacggtg tggtccattg gctactggct ctgctacgtc aacagcacca tcaaccctgc
     4921 ctgctatgct ctgtgcaacg ccacctttaa aaagaccttc cggcacctgc tgctgtgcca
     4981 gtatcggaac atcggcactg ccaggcggga tccaccggtc gccaccatgg tgagcaaggg
     5041 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     5101 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5161 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     5221 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5281 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5341 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5401 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     5461 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5521 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5581 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5641 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     5701 ccactccacc ggcggcatgg acgagctgta caagtaaggc gcgccacgcg tgaattcgat
     5761 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5821 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5881 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5941 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6001 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6061 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6121 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6181 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6241 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6301 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6361 ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc
     6421 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     6481 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     6541 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     6601 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     6661 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     6721 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     6781 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     6841 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc aggcgcgtga
     6901 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     6961 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     7021 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     7081 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     7141 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     7201 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     7261 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     7321 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     7381 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     7441 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7501 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7561 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7621 cgtgccagcg gatcgacagt actaagcttt actagggaca ggattggtga cagaaaagcc
     7681 ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc cacctcctgt
     7741 taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc tctccttgcc
     7801 agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg agagcttggc
     7861 agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg ccaccctgcg
     7921 ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt tcactgatcc
     7981 tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa acaaaatcag
     8041 aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc aatttatatt
     8101 gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc cgtcctggca
     8161 gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga atggtgcgtc
     8221 ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca tccttctttc
     8281 cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag ggtcccgctt
     8341 ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag gagaggcgct
     8401 caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct cctgcccctt
     8461 ccctacaggg gttcctggct ctgctctaag ggcaagggcg aattcgcggc cgctaaattc
     8521 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     8581 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8641 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac
     8701 gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     8761 gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca
     8821 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     8881 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     8941 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     9001 ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg
     9061 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     9121 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     9181 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     9241 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     9301 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
     9361 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     9421 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     9481 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     9541 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     9601 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     9661 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     9721 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     9781 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     9841 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     9901 gaggatctcg tcgtgaccca tgcatggcga tgcctgcttg ccgaatatca tggtggaaaa
     9961 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
    10021 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    10081 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    10141 tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac
    10201 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
    10261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa
    10321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11281 aatattattg aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt
    11341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    12061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    12121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    12181 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    12241 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    12301 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12361 acacaggaaa cagctatgac catgattacg ccaagctcag aattaaccct cactaaaggg
    12421 t
//
