LOCUS       sequence-156453-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of DAPK1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: DAPK1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca accgtgttca ggcaggaaaa cgtggatgat tactacgaca
      181 ccggcgagga acttggcagt ggacagtttg cggttgtgaa gaaatgccgt gagaaaagca
      241 ccggcctcca gtatgccgcc aaattcatca agaaaaggag gactaagtcc agccggcggg
      301 gtgtgagccg cgaggacatc gagcgggagg tcagcatcct gaaggagatc cagcacccca
      361 atgtcatcac cctgcacgag gtctatgaga acaagacgga cgtcatcctg atcttggaac
      421 tcgttgcagg tggcgagctg tttgacttct tagctgaaaa ggaatcttta actgaagagg
      481 aagcaactga atttctcaaa caaactctta atggtgttta ctacctgcac tcccttcaaa
      541 tcgcccactt tgatcttaag cctgagaaca taatgctttt ggatagaaat gtccccaaac
      601 ctcggatcaa gatcattgac tttgggttgg cccataaaat tgactttgga aatgaattta
      661 aaaacatatt tgggactcca gagtttgtcg ctcctgagat agtcaactat gaacctcttg
      721 gtcttgaggc agatatgtgg agtatcgggg taataaccta tatcctccta agtggggcct
      781 ccccatttct tggagacact aagcaagaaa cgttagcaaa tgtatccgct gtcaactacg
      841 aatttgagga tga
//
