LOCUS       sequence-156457-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of SLK. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: SLK_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca aagcagtacg aacacgtgac gagggacctg aaccccgaag
      181 acttttggga gattatagga gaactgggcg acggagcctt tgggaaagtg tacaaggccc
      241 agaataaaga gaccagtgtt ttagctgctg caaaagtgat tgacactaaa tctgaagaag
      301 aacttgaaga ttacatggta gagattgaca tattagcatc ttgtgatcac ccaaatatag
      361 tcaagcttct agatgccttc tattatgaga acaatctttg gatcctcatt gaattttgtg
      421 caggtggagc agtagatgct gtgatgcttg aacttgagag accattaact gagtcccaaa
      481 tacaagtagt ttgcaagcag actttagatg cattgaacta cttacatgat aataagatca
      541 tccacagaga tctgaaggct ggcaacattc tctttacctt agatggagat atcaaattgg
      601 cggattttgg agtatcagct aaaaacacga ggacaattca aagaagagat tcctttattg
      661 gtacaccata ttggatggct cctgaagtag tcatgtgtga aacatctaag gacagaccct
      721 atgactacaa agctgatgtt tggtccctgg gtatcacttt aatagaaatg gctgagatag
      781 aaccacctca tcatgaatta aatccaatgc gagtgctgct aaaaatagca aaatctgagc
      841 cacctacatt agcacagcca tccagatg
//
