LOCUS       sequence-156656-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector under the CMV promoter that expresses 
            smURFP and mCardinal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pLenti-smURFP-T2A-mCardinal
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..395
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="KTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAFSQ
                     RDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRCLK
                     EASLSLLDEEDANETAPYFDYIIKAMS"
     CDS             423..476
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ctaagacttc cgaacagagg gtgaacattg ctacactgct gacagaaaat aagaagaaaa
       61 tcgtggataa ggcttcccag gatctgtggc ggagacaccc agacctgatc gcaccaggag
      121 gaattgcttt ctctcagagg gaccgcgctc tgtgcctgcg agattacggc tggttcctgc
      181 atctgatcac cttttgtctg ctggccggag ataagggccc catcgagtct attgggctga
      241 tcagtattcg agaaatgtat aactcactgg gagtgcccgt ccctgcaatg atggagagca
      301 ttagatgcct gaaagaagcc agcctgtccc tgctggacga agaggacgcc aacgagaccg
      361 caccctactt tgattacatt attaaggcta tgagcgctag cggaggtgga tctggtggcg
      421 gtgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctcgta
      481 cgatggtgag caagggcgag gagctgatca aggagaacat gcacatgaag ctgtacatgg
      541 aaggcaccgt gaacaaccac cacttcaagt gcaccaccga aggggagggc aagccctacg
      601 agggcaccca gacccagagg attaaggtgg tggagggagg ccccctgccg ttcgcattcg
      661 acatcctggc cacctgcttt atgtacggga gcaagacctt catcaaccac acccagggca
      721 tccccgattt ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
      781 acgaagacgg gggcgtgctt accgttaccc aggacaccag cctccaggac ggctgcttga
      841 tctacaacgt caagctcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga
      901 aaacactcgg ctgggaggcc accaccgaga ccctgtaccc cgctgacggc ggcctggaag
      961 gcagatgcga catggccctg aagctcgtgg gcgggggcca cctgcactgc aacctgaaga
//
