LOCUS       sequence-156702-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of hM4Di-mCherry under CBA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Hou XH, Hyun M, Taranda J, Huang KW, Todd E, Feng D, Atwater E, 
            Croney D, Zeidel ML, Osten P, Sabatini BL
  TITLE     Central Control Circuit for Context-Dependent Micturition.
  JOURNAL   Cell. 2016 Sep 22;167(1):73-86.e12. doi: 10.1016/j.cell.2016.08.073.
  PUBMED    27662084
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "73-86.e12"
COMMENT     Sequence Label: pAAV-CBA-DIO-hM4Di-mCherry
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(118..151)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(438..457)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 attggtaccg gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
       61 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
      121 acttcgtata gcatacatta tacgaagtta tggcgcgcct tacttgtaca gctcgtccat
      181 gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc
      241 gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg
      301 cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt
      361 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
      421 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
      481 gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt
      541 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
      601 cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtacat
      661 gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg tcaccttcag
      721 ct
//
