LOCUS       sequence-156718-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused 
            to Cdt1 peptide and is degraded during S/G2 phases of the 
            cell-cycle..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, 
            Elefanty AG, Stanley EG
  TITLE     A Cas9 Variant for Efficient Generation of Indel-Free Knockin or 
            Gene-Corrected Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 
            10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
  PUBMED    27499201
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2016.07.001"; journalName: "Stem Cell Reports"; 
            date: "2016-09-13- 13"; volume: "7"; issue: "3"; pages: "508-17"
COMMENT     Sequence Label: pDNR-SpCas9-Cdt1
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagaggatcg aacccttgcc accatggaca agaagtactc cattgggctc gatatcggca
       61 caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca
      121 aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt
      181 tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata
      241 cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg
      301 tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc
      361 acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc
      421 caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt
      481 tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg
      541 acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca
      601 atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga
      661 gcgctaggct gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga
      721 agaagaacgg cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta
      781 aatctaactt cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg
      841 atgatctcga caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg
      901 caaagaacct gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca
      961 ccaaagctcc gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga
     1021 ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg
     1081 atcagtctaa aaatggctac gccgga
//
