LOCUS       sequence-156751-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused 
            to Cdt1 peptide and is degraded during S/G2 phases of the 
            cell-cycle..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, 
            Elefanty AG, Stanley EG
  TITLE     A Cas9 Variant for Efficient Generation of Indel-Free Knockin or 
            Gene-Corrected Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 
            10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
  PUBMED    27499201
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2016.07.001"; journalName: "Stem Cell Reports"; 
            date: "2016-09-13- 13"; volume: "7"; issue: "3"; pages: "508-17"
COMMENT     Sequence Label: pDNR-SpCas9-Cdt1
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..302)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
ORIGIN
        1 gtttaaactc attactaacc ggtagattag atggtgtcct ggtcctgcgc ggatgtcagg
       61 tggggcggct gacctgctgc cggggtggat ttctttatct tctggcccgg agaagggcag
      121 gctgggatgt ctggggcctc gggggtactg gggctggaaa cctcgtccac cgacagccgc
      181 agtctcctgc gggccggtgg cctggcctgg tcgcgtccgg gggcggcggg cgggcgggcg
      241 cgcttgcggc tgccactggt agcggaggcc ggggcgcgga gtgcgggcct ggcggggctg
      301 ggctcgagcg gccgccagtg tgatggatat cccttgtcag ccctgctgtc tccaccgagc
      361 tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat cagtgtggcg
      421 tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc gaagtacttg
      481 aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt ttctgcctgc
      541 tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc gaggttagcg
      601 tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc atcaaggtag
      661 tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga ccctttgagc
      721 ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag tgccagctcg
      781 ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt ttcaagctca
      841 aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt atatcctttc
      901 gcctcgagaa agtcgatggg gttttt
//
