LOCUS       sequence-156757-        7033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expressing human TRF1 with N-terminal MYC tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Zimmermann M, Kibe T, Kabir S, de Lange T
  TITLE     TRF1 negotiates TTAGGG repeat-associated replication problems by 
            recruiting the BLM helicase and the TPP1/POT1 repressor of ATR 
            signaling.
  JOURNAL   Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. 
            Epub 2014 Oct 24.
  PUBMED    25344324
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.251611.114"; journalName: "Genes Dev"; date: 
            "2014-11-15- 15"; volume: "28"; issue: "22"; pages: "2477-91"
COMMENT     Sequence Label: pLPC-MYC-hTRF1
FEATURES             Location/Qualifiers
     source          1..7033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     866..885
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        866..884
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      916..925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             925..954
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     LTR             2510..2979
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3109..3126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3280..3868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3360..3379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4028..4819,4820..4888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4651..4670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4889..4993)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5024..5493
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5558..5757
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5958..6374
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6304..6326
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6342..6358
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6392..6991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6392..6411)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6888..6908
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgaccccatg catcggatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
      841 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
      901 accgagctcg gatcagccac catggaacaa aagttgattt ctgaagaaga tttgttaaga
      961 aagagaggat ccgcggagga tgtttcctca gcggccccga gcccgcgggg ctgtgcggat
     1021 ggtagggatg ccgaccctac tgaggagcag atggcagaaa cagagagaaa cgacgaggag
     1081 cagttcgaat gccaggaact gctcgagtgc caggtgcagg tgggggcccc cgaggaggag
     1141 gaggaggagg aggaggacgc gggcctggtg gccgaggccg aggccgtggc tgccggctgg
     1201 atgctcgatt tcctctgcct ctctctttgc cgagctttcc gcgacggccg ctccgaggac
     1261 ttccgcagga cccgcaacag cgcagaggct attattcatg gactatccag tctaacagct
     1321 tgccagttga gaacgatata catatgtcag tttttgacaa gaattgcagc aggaaaaacc
     1381 cttgatgcac agtttgaaaa tgatgaacga attacaccct tggaatcagc cctgatgatt
     1441 tggggttcaa ttgaaaagga acatgacaaa cttcatgaag aaatacagaa tttaattaaa
     1501 attcaggcta tagctgtttg tatggaaaat ggcaacttta aagaagcaga agaagtcttt
     1561 gaaagaatat ttggtgatcc aaattctcat atgcctttca aaagcaaatt gcttatgata
     1621 atctctcaga aagatacatt tcattccttt tttcaacact tcagctacaa ccacatgatg
     1681 gagaaaatta agagttatgt gaattatgtg ctaagtgaaa aatcatcaac ctttctaatg
     1741 aaggcagcgg caaaagtagt agaaagcaaa aggacaagaa caataacttc tcaagataaa
     1801 cctagtggta atgatgttga aatggaaact gaagctaatt tggatacaag aaaaagtgtt
     1861 agtgacaaac agtctgcggt aactgaatcc tcagagggta cagtatcctt attgaggtct
     1921 cacaagaatc ttttcttatc taagttgcaa catggaaccc agcaacaaga ccttaataag
     1981 aaagaaagaa gagtaggaac tcctcaaagt acaaaaaaga aaaaagaaag cagaagagcc
     2041 actgaaagca gaatacctgt ttcaaagagt cagccggtaa ctcctgaaaa acatcgagct
     2101 agaaaaagac aggcatggct ttgggaagaa gacaagaatt tgagatctgg cgtgaggaaa
     2161 tatggagagg gaaactggtc taaaatactg ttgcattata aattcaacaa ccggacaagt
     2221 gtcatgttaa aagacagatg gaggaccatg aagaaactaa aactgatttc ctcagacagc
     2281 gaagactgag aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     2341 gggccgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     2401 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     2461 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     2521 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     2581 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     2641 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     2701 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     2761 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     2821 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     2881 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     2941 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     3001 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     3061 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3961 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     4021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4621 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4981 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac
     5041 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     5101 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     5161 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     5221 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     5281 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     5341 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     5401 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     5461 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     5521 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt
     5581 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     5641 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
     5701 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
     5761 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
     5821 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
     5881 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     5941 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     6001 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     6061 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     6121 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     6181 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     6241 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     6301 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     6361 cttctctagg cgccggccgg atcagcttac catgaccgag tacaagccca cggtgcgcct
     6421 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     6481 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     6541 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6601 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6661 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6721 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6781 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6841 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6901 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6961 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     7021 cgaaaggagc gca
//
