LOCUS       sequence-156786-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driven sgRNA expression plasmid. Annealed 
            oligonucleotides encoding crRNA sequence are ligated into BbsI 
            site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, 
            Elefanty AG, Stanley EG
  TITLE     A Cas9 Variant for Efficient Generation of Indel-Free Knockin or 
            Gene-Corrected Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 
            10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
  PUBMED    27499201
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2016.07.001"; journalName: "Stem Cell Reports"; 
            date: "2016-09-13- 13"; volume: "7"; issue: "3"; pages: "508-17"
COMMENT     Sequence Label: pSMART-sgRNA (Sp)
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..131
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(143..730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(223..242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(752..781)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     890..910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact
       61 tgatgctcga tgagtttttc tgagggccca aatgtaatca cctggctcac cttcgggtgg
      121 gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      181 atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      601 gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat ccggcaaaca
      661 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      721 aggatctcaa gaagatcctt tgattttcta ccgaagaaag gcccacccgt gaaggtgagc
      781 cagtgagttg attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa
      841 gcttgaatcc aagctcggat ccgatatcga cgtcaaggtc gggcaggaag agggcctatt
      901 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      961 taatttgact gtaaacacaa agatattagt acaaaatac
//
