LOCUS       sequence-156845-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of plasma-membrane anchored Zdk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-NLyn-Venus-Zdk1
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(174..197)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(204..236)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(276..299)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(306..326)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(333..350)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(369..545)
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
     CDS             complement(552..803)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(594..615)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     943..962
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
       61 taatttttgg cagagggaaa aagatcgatc tcagtggtat ttgtgagcca gggcattggc
      121 cacaccagcc accaccttct gataggcagc ctgcacctga ggttaatcac ttagtgatgg
      181 tgatggtgat ggtggtgctc gagatcctcg gggtcttccg gggcgagttc tggctggctt
      241 gcacgtgtat acagctgtgc ggccgcaagc ttttatttgt cgtcgtcgtc cttgtagtcg
      301 cggccgccct ggaaatacag attttcaccg ccatgatgat gatgatgatg gctaccacca
      361 gaaccacctt ttggggcctg ggcatcgttc agttttttgg cttcggccag cagattggcg
      421 ctctgagatg gatcatcaaa caggctcacg atgaaggcaa acttctgctc aacattcagg
      481 tttggcagag aatggatttc cgcaccggca cgcgtctttt ctttattgaa tttgttatcc
      541 accatggatc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      601 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagttt ggactggtag
      661 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      721 tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt gaagtttgcc
      781 ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgtt gtagttgtac
      841 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      901 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      961 tccttgaaga agatggtgcg ctcctggacg ta
//
