LOCUS       sequence-156987-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Weinl C, Marquardt S, Kuijt SJ, Nowack MK, Jakoby MJ, Hulskamp M, 
            Schnittger A
  TITLE     Novel functions of plant cyclin-dependent kinase inhibitors, 
            ICK1/KRP1, can act non-cell-autonomously and inhibit entry into 
            mitosis.
  JOURNAL   Plant Cell. 2005 Jun;17(6):1704-22. Epub 2005 Mar 4.
  PUBMED    15749764
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell."; date: "2005-06"; volume: "17(6)"; pages: "1704-22. Epub 2005
            Mar 4"
COMMENT     Sequence Label: pAM-PAT-ProGL2 GW
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..26
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(120..372)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(392..943)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(985..1164)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    3439..3563
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3588..3593,3594..3611,3612..3618)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3672..4331
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3738..3757)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4673..4978
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4857..4876
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5019..5143)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5514..5538
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5650..6359
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6420..6524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6525..6593,6594..7385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6743..6762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7556..8144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8045..8064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
       61 ggacgttttt aatgatcgaa tactaacgtc tctaccagat atcagcttgc atgccggtcg
      121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      361 gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc aggaccggac
      421 ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
      481 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
      541 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
      601 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
      661 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac
      721 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
      781 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
      841 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
      901 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat cccctcgatc
      961 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     1021 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     1081 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
     1141 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     1201 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
     1261 ccgccttcgg tttgggcgcg ccaagctttt gaattgtaga taaatcatct gctacagtta
     1321 taccattata tatcttatta aagacctaag tttccttcac tatacgtctt cgtccattta
     1381 cgtacgtatt atacggacgg tttaagctac tatatctata ttgttaacaa tgtaactgtt
     1441 gagatatatc ttgcaataat atgtcatggt gtatgcatac gataatatga atcaatgttt
     1501 gaaatcttga cgtgcccgtg atacaataag atgatcaaaa tttcaaattt tgtcaaatat
     1561 taaaacaaca tacacataca catgtgtcca ggtggcatta taaaatgtat atatggtgga
     1621 tatagagaga gagggagatg cgtatagtga ataggaaagt aagtaataaa gagagggtgg
     1681 aggaattgga aaggggttgg aggcaaaccc ataaagagca ttcatttcct tttaaggtcg
     1741 ctgaaattaa tgagtaacga tcggtcaatg cctctcgctg acctttttct ttttttacaa
     1801 caacaaataa aaataaaata aatttcgacg tctctttccg ctgctgaatt acatttgttg
     1861 aattaatttt ctctgcttac gtacgtcttc taaactttct ctatccgaat tcttttttaa
     1921 ctttctaact tatattcaac aactcttctt tcctgccttt accgttagtc taattgtttt
     1981 cctaatactg ctacgtacat acccctacta tactagtcag tgtattagat tcgattggga
     2041 ttaatccagg aatatagata tcccattagt ttttataaaa atattggaag aggacaagtc
     2101 tcaagcaatt tagggttcca tgtagcgctg caatatactg ttagtaactc tctcttaccc
     2161 atatattgta tatgctaatt cttatcaaat atatatatat gcttctccca gagtcccagt
     2221 ttcctataat cctgacgcaa ttatactaat agagccaagt ttacataata aagtatatat
     2281 gattaataga tagggtttct tattaagcca tatcttaaat taagatgtga tgatagcgtt
     2341 ttgtataagt taccaattgt ttgaaagaag agatcatcac aataataaat cataagtagt
     2401 agtatatagt aataaataaa tacacaagtc ataataagag taatgagagg ataattaagg
     2461 agggaagaag aaagcagaaa atgcggttgg agaattaggt gctaaaagtt agttgagtcc
     2521 atctcagtat ctaacggtca actctctctc tctctagaga aaacaattaa gaaatctgac
     2581 atacacatat gtctctctct ctctctctct ctagtctata cacacaattc aattaaagaa
     2641 gagacagaga agttcgtctt ttttgttttt atacccttaa atcaatcatg caattgtaac
     2701 ccttccttct tattctcatt ccttcccccc ctgtctacag taatctatag caacgccatt
     2761 atgtactact tttaacggat aatttgctca tgtttcaata tggcttcact gtatatatgt
     2821 tcaagttctt ctcaatccct ttatatcatt ccaacataat tcatattaaa gttagtagct
     2881 gaaattggaa ggctgatata ttttccataa ttcaaatttg aattttgctc atcatatata
     2941 tatgtatata ttaaaaatcg aatattaaga agaaaaatga agtcgatcga tggctgccaa
     3001 tgctgtagct ggccatgttt taaactactc aattgtcgga ttgaagtata gccaaaatat
     3061 ataaaaccgt aaaaggacta aatataataa tataataggt attaattaat taaaactaat
     3121 taattataaa agaagcacct aaaagtcaag agcagtagag aaatggaaga aatatctgaa
     3181 aaacgaccgc ttatatatat atgtatcatt ggaattgaag aggctatata tatatatata
     3241 tatatatata tatatatatc gatcttagct tatatattaa ttgaaagtac attttggtgt
     3301 ataagtaatt aaagaagaaa gaaaaaaaga gagataatat ataaggaaga aggagtgcga
     3361 ggagaagagg gaagagatca taattaagca aagaagctag aggatccttg ctcgagaaag
     3421 aggatccacc tgaggatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
     3481 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     3541 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
     3601 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     3661 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     3721 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     3781 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     3841 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     3901 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     3961 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     4021 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     4081 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     4141 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     4201 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
     4261 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     4321 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     4381 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     4441 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     4501 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     4561 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     4621 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt
     4681 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     4741 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     4801 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     4861 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     4921 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     4981 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     5041 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     5101 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatcctc cgttccatgg
     5161 gctagaagct tgttgagaat tcatcgcccg ggactgggtt aacttccgat cgattctaga
     5221 ctagttctag agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt
     5281 ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt
     5341 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     5401 tatcaataaa atttctaatt cctaaaacca aaatccagtg accgggcggc cgccaccgcg
     5461 gtggaggggg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
     5521 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     5581 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg tacatcaccg acgagcaagg
     5641 caagaccgag cgcctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg
     5701 ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccggc
     5761 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
     5821 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
     5881 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
     5941 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
     6001 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
     6061 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
     6121 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
     6181 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
     6241 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
     6301 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
     6361 gctggcagtc cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgac
     6421 aagctacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7681 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8161 cctttttacg gttcctggcc ttttgctggc cttttgctca catggactct agctagagga
     8221 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     8281 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     8341 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     8401 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctg
//
