LOCUS       sequence-157012-        7853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Ple266 (S100B MiniPromoter) 
            insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1943
FEATURES             Location/Qualifiers
     source          1..7853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3243..3262)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3329..3420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3421..3489,3490..4281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3639..3658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(5166..5185)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5901..6434
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      6556..7144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7045..7064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7298..7315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7413..7430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7414..7430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccttgctgca
       61 ggcgtcgtct ctgctgcggg tcctgggcgg caggactggt gggcagaggc agcctcttat
      121 tccctctgcc cagggaggat ggatgaaccc tggtgcagcc cactgcaggc cgtggcagcc
      181 aatgggagcc gaaggcgggg gtggcagggc tgccaggcct gcacacaggg ctgcattgac
      241 caagcagccc cggggacttg ctgggagcca ggattgcaca tgggctgagg aagaaaaaag
      301 ggcctgtagg gcaggaagtg gggagtcagc tttttgtgcg atttctttat tacatgaata
      361 ttgatgtcac cctaaaggca aggttggggg tggggagagg agagagactt ggattaattg
      421 attcaaacaa agcaaatgtg cagccaagaa caagcagctt ccaagtacag gctgagcata
      481 ggagattaag tggcctgcaa gttggtttct aatggagagg cccctggagc ttctctcctc
      541 tgccatcctc caagtctggc cccactgggc gtgaagttct gtggacagta gtgcttgtct
      601 gtggtcagcc ctgggctcaa cggggctgcc tcagagaagc tgctgaggcc agtgtctgca
      661 gggactaaaa cgggtggtac ttttagggga aaacgtgatg tgtggactgt atcccaaggc
      721 ctttccctcc accccagggt ggaaggtcac agactctcag agttggacga tgccttagag
      781 atgtcccgtt cgaacgctgt tttacagagg cgtgtggacg gtgtgccccc ttgctgaagc
      841 ctccagccag cctgaggaag gagaagtcca gctgtcccct caactcgctc gactggagcc
      901 tctgaacacg ggggccagtt tatgggttct tcttcacaag aatgaagaag taggtgttcc
      961 ttctgacaaa ttacacaaga acattggccc aaactctcca aaaatgtcca tggtccccaa
     1021 tggatgcctg acccagcatc acgtccttct cccaacccag tttcaaagtg gccgtgaaag
     1081 ggtttcccac aagatatctg tgtacatggc tgtcttgctg tcattgtttt atccaggacg
     1141 ttctctacca ttcaggatct gtaagtgtgc cctggaaata gcacagggct gagagctgtg
     1201 gtcttgcgtg gctcagcctg ccccagcctc ctgtgaggaa cagcctgagc agtgtggttg
     1261 gccctcactt cctcatcggc ttcaccaccc ctggctgtgg tctctgctga aggatctgac
     1321 acaggcttgc tctgtgaagc acagagcttg cccaggccca aggaaggcac aactgtgctc
     1381 tgtgcccctt cctcgctcag atgttggagg gaccattcct gtaagtgctg accacgctgc
     1441 ctgcccaggc cagagcacga aggagcttgt gggtcaacag gggcctctat ttgaatatat
     1501 caatcttctg ttaagatctt cttcagtcac ttggatgtcc cttctccctt catccattca
     1561 gcaaactctt ggctccaatc aactaggcag ccagccccgg gccttgctgg ggctggtcag
     1621 ggggtgggaa ggggtcattc cagaaaggcc tgggcagcaa cattgttttc tcccacaaag
     1681 gacggtgcca tccgccacca agccactcac gaggctgact ccagtgaccg atggccacag
     1741 gcagaaaagg aggcttgtgt tctaaaggaa tgtgaggaag agacaaaaag cttctgttgt
     1801 agcaaatgca agtattgatg caatgagaac tcactgcctt tcctgttcta aagaatgcca
     1861 ctcccagcca gcaggcagcc atggggtcag ggcttgggtg ctggggcctt ggagctggat
     1921 tcagatccga cctctgaaca aaccttgtca aacgacaacc tttctcaggc tgagtaaaag
     1981 acagaaaaaa agctgcaccc ctgagagggc tgtaggagga gtaaaggaaa ttgacacgca
     2041 catacatgtg cacacataca gacacacgtg aagggcccaa tgcactcact tgggagtcat
     2101 tattattatt ataaaagtta ctgttcttgt ctgcagaacc tggagctggc aggagtcaga
     2161 ctgggcaaag caaagaacag caaatagcag agggacagag acttctttct gagccagata
     2221 tattaggtct aaaagggcag agtgaaccta ctggctactt tagaatactc taaatccagg
     2281 aggcccccaa tctggcaaat taaggcagaa tatcagcatt tcatgggtat cacattttat
     2341 tatttttgaa ctatgtcaaa ttccattaat tcatttgggt tctaaccagt cattccaaag
     2401 tccatgctcc cttccagaag tgcaggccca gcatgagctg agccgggggc agagcaggag
     2461 caccgctgtt tgccctgggt ccatggcaac gccagagccc agctcaacac agcagggctt
     2521 ttgttttcat ttttcaactt tttttgaggc cttgagaagg aagtggagat tggagtgtga
     2581 agagcccacc ccacacaaag ccgcttttca tggcacttgc tgtgcctgtg gtttcttgct
     2641 caggaactga gtcttggtga aatatatgag gaagtgtttg aactgatgct ggggcaagat
     2701 ctcaaaagga aaactgagtt ttcccttttg tagaactctg tcacagatat ttattttgat
     2761 tatgcttttt ataactttta aggattcttg gaaatttgca ataaaatcat gtcaggttta
     2821 aatgtttaaa aaatcatagg attagcctat ttgttttttc actcaacagg agtgtttttc
     2881 atttgacgac ttcagaagat cccacgtctt acagcccgtc ccttcctgta ggacgctgtg
     2941 ggccaggaca gcacagtact cggccacatg cccaaatgag cagccacaaa agtctaatca
     3001 atcgattgtt caaactgctt aactcacatc tgacgcgtcc ccgagtcgaa taagggcgac
     3061 acccctcaat tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg
     3121 atgcctggca gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc
     3181 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca
     3241 aacaaggggt gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt
     3301 ggttaattgg ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg
     3361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat
     3421 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3481 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3541 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3601 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3661 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3721 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3781 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3841 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3901 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3961 cctgtagcga tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4021 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4081 tcggcccttc cggctggctg gtttattgct gataaatccg gagccggtga gcgtggttct
     4141 cgcggtatca tcgcagcgct ggggccagat ggtaagccct cccgtatcgt agttatctac
     4201 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4261 tcactgatta agcattggta aaggaggaaa aaaaaatgag ccatattcaa cgggaaacgt
     4321 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     4381 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     4441 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     4501 gactaaactg gctgacggaa tttatgccac ttccgaccat caagcatttt atccgtactc
     4561 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcgttc caggtattag
     4621 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgtgc acctgtttgt
     4681 aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc aggcgcaatc acgaatgaat
     4741 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     4801 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     4861 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     4921 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     4981 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     5041 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaaaggag gaaaaaaaaa
     5101 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     5161 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     5221 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     5281 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
     5341 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag tgcacacgac
     5401 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     5461 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tcagcgccaa tccctgggtg
     5521 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     5581 actatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     5641 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     5701 tgcgatgagt ggcagggcgg ggcgtaaacg ccgaggagga aaaaaaaatg cgctcacgca
     5761 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     5821 cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     5881 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     5941 gccctaaaac aaagttaggt ggctcaagta tgggcatcat tcgcacatgt aggctcggcc
     6001 ctgaccaagt caaatccatg cgggctgctc ttgatctttt cggtcgtgag ttcggtgacg
     6061 tagccaccta ctcccaacat cagccggact ccgattacct cgggaacttg ctccgtagta
     6121 agacattcat cgcgcttgct gccttcgacc aagaagcggt tgttggcgct ctcgcggctt
     6181 acgttctgcc caagtttgag cagccgcgta gtgagatcta tatctatgat ctcgcagtct
     6241 ccggcgagca ccggaggcag ggcattgcca ccgcgctcat caatctcctc aagcatgagg
     6301 ccaacgcgct tggtgcttat gtgatctacg tgcaagcaga ttacggtgac gatcccgcag
     6361 tggctctcta tacaaagttg ggcatacggg aagaagtgat gcactttgat atcgacccaa
     6421 gtaccgccac ctaagcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     6481 aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa
     6541 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6601 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6661 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6721 gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6781 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6841 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6901 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6961 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7021 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7081 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7141 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7201 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7261 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7321 ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc tgacggatgg
     7381 cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt cttaagctcg
     7441 ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
     7501 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
     7561 ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg agaaaaaagc
     7621 aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata attaaactat
     7681 tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa agagaattaa
     7741 gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa attatacatg
     7801 tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt att
//
