LOCUS       sequence-157115-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector encoding non-targeting shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, 
            Stokke T, Patzke S, Saunders DN
  TITLE     The E3 ubiquitin ligase UBR5 regulates centriolar satellite 
            stability and primary cilia.
  JOURNAL   Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 
            10.1091/mbc.E17-04-0248.
  PUBMED    29742019
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248."
COMMENT     Sequence Label: pEN_TmiRc3_shNT
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(147..273)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(302..316)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(345..466)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(608..632)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(629..667)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(700..718,719..741,742..760,761..783,784..802,803..825,
                     826..844,845..867,868..886,887..909,910..928,929..951,
                     952..970)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggtct agatatctcg
      121 aaaacgcatt agtcttccaa ttgaaaaaag tgatttaatt tataccattt taattcagct
      181 ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt tagtaaacaa gataattgct
      241 cctaaagtag ccccttgaat tccgaggcag taggcagcga tgctctaagg ttctatcaat
      301 acatctgtgg cttcactatt gatagaacct tagagcatcg acgctcactg tcaacagcaa
      361 tataccttct cgagccttct gttgggttaa cctgaagaag taatcccagc aagtgtttcc
      421 aagatgtgca ggcaacgatt ctgtaaagta ctgaagcctc attcaaacaa ttaccctgtt
      481 atccctagtc gactaggact agagtcacta gtgatcagac gcctaggcct agttcggggc
      541 cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc
      601 tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc caccgtacac
      661 gcctacctcg acccgggtac cgagctcgac tttcactttt ctctatcact gatagggagt
      721 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
      781 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
      841 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      901 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
      961 ctgataggga gtggtaaagg atccagtcga ctgaattggt tcctttaaag cctgcttttt
//
