LOCUS       sequence-157127-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #3-RFP
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      161..232
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      248..275
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(303..320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(437..984)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(517..536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctaaaccgg ttcgctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
       61 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
      121 gtgcttaagg atccaaactc gagtaaggat ctccaggcat caaataaaac gaaaggctca
      181 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      241 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat acctagggat atattccgct
      301 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
      361 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
      421 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
      481 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      541 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
      601 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
      661 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
      721 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
      781 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
      841 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
      901 gagaaccttc gaaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
      961 cgcgcagaac caaaacgatc tcaa
//
