LOCUS       sequence-157150-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express PAsfCherry2(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pcDNA3.1_PAsfCherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggaggagg ataacatggc catcattaag gagttcatgc gcttcaaggt gcacatggag
       61 gggtccgtga acggccacgt gttcgagatc gagggcgagg gcgagggcca cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cacctgggac
      181 atcctgtccc ctcaattcat gtacggctcc aatgcctacg tgaagcaccc cgccgacatc
      241 cccgactact ttaagctgtc cttccccgag ggcttcacct gggagcgcgt gatgaaattc
      301 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg tcagttcatc
      361 tacaaggtga agctgctggg catcaacttc ccctccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggccct caccgagcgg atgtaccccg aggacggcgc cctgaagggc
      481 gaggtcaacc cgagagtgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      541 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cacacaacga ggactga
//
