LOCUS       sequence-157306-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving Avi-tagged protein containing Cerulean 
            protein fused to the carboxy-terminal domain of avian Ran 
            GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-Avi-Cerulean-RanGAP
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(594..845)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(636..657)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccggttaata cgactcacta tagggagtcc ggatacgtat gaattcatta ttacagctgg
       61 tggagggttt gtaaaagcat gtggcgggca aaggaacagg agtcgagagc acggtttggt
      121 tttgtgacaa aagcagagag aactggggcc agtgatttag ggaaataatc ctgctggacc
      181 atatgattca aagtcataag aataccatag aggctcggga cagctttgat cttctcttca
      241 ctcttaagta gccccatgtg gatcaagagt cttgtgatga atgcatctga attaaatgtg
      301 gcggaagaga aggctttttt catcaaggca tctgctgttt catgcactgc tgtttttact
      361 tgggcttcat ctttgaagac tgaagatatc cttaagagag ctgtaactac tttctctaca
      421 tcagatgtat ctgtctgctg agctatcagc acagaacact ttggccctag ccgcagcagc
      481 ttctctggtg atgggaaagc aaggaacgtg gcaacgtcca caggaggaga tggcactgga
      541 gttgaatcct gtgaaccaat tatcttctta ggagccataa tcgatgcagc ggccttgtac
      601 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      661 tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg
      721 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      781 acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc
      841 ttgtcggcgg tgatatagac gttgtcgctg atggcgttgt actccagctt gtgccccagg
      901 atgttgccgt cctccttg
//
