LOCUS       sequence-157314-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), mCherry protein and SV40 polyA tail, 
            followed by FRT site-flanked Kanamycin selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..58
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             65..91
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ccggccgcca tggcggccgc ggatggccac ctatgacgtc ccagattacg caagtttgcc
       61 cggatatgat gttcctgatt atgctagcct cccgggttac gatgtgcccg actatgcctc
      121 acttccaggc agatctccta tgaaggataa caccgtgcca ctgaaattga ttgccctgtt
      181 agcgaacggt gaatttcact ctggcgagca gttgggtgaa acgctgggaa tgagccgggc
      241 ggctattaat aaacacattc agacactgcg tgactggggc gttgatgtct ttaccgttcc
      301 gggaaaagga tacagcctgc ctgagcctat ccagttactt aatgctaaac agatattggg
      361 tcagctggat ggcggtagtg tagccgtgct gccagtgatt gactccacga atcagtacct
      421 tcttgatcgt atcggagagc ttaaatcggg cgatgcttgc attgcagaat accagcaggc
      481 tggccgtggt cgccggggtc ggaaatggtt ttcgcctttt ggcgcaaact tatatttgtc
      541 gatgttctgg cgtctggaac aaggcccggc ggcggcgatt ggtttaagtc tggttatcgg
      601 tatcgtgatg gcggaagtat tacgcaagct gggtgcagat aaagttcgtg ttaaatggcc
      661 taatgacctc tatctgcagg atcgcaagct ggcaggcatt ctggtggagc tgactggcaa
      721 aactggcgat gcggcgcaaa tagtcattgg agccgggatc aacatggcaa tgcgccgtgt
      781 tgaagagagt gtcgttaatc aggggtggat cacgctgcag gaagcgggga tcaatctcga
      841 tcgtaatacg ttggcggcca tgctaatacg tgaattacgt gctgcgttgg aactcttcga
      901 acaagaagga ttggcacctt atctgtcgcg ctgggaaaag ctggataatt ttattaatcg
      961 cccagtgaaa ctt
//
