LOCUS       sequence-157391-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cas9 and two gRNA cassettes in S. cerevisiae; 
            Nourseothricin Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Generoso WC, Gottardi M, Oreb M, Boles E
  TITLE     Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
  JOURNAL   J Microbiol Methods. 2016 Aug;127:203-5. doi: 
            10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
  PUBMED    27327211
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods."; date: "2016-08"; volume: "127:203-5. doi"; pages: " 
            10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17"
COMMENT     Sequence Label: pDuRCC-N
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..565
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLR
                     EVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLT
                     VEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCG
                     LDTALYDGTASDGEQALYMSMPCP"
     terminator      571..768
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cactcttgac gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat
       61 cgaggcactg gatgggtcct tcaccaccga caccgtcttc cgcgtcaccg ccaccgggga
      121 cggcttcacc ctgcgggagg tgccggtgga cccgcccctg accaaggtgt tccccgacga
      181 cgaatcggac gacgaatcgg acgacgggga ggacggcgac ccggactccc ggacgttcgt
      241 cgcgtacggg gacgacggcg acctggcggg cttcgtggtc gtctcgtact ccggctggaa
      301 ccgccggctg accgtcgagg acatcgaggt cgccccggag caccgggggc acggggtcgg
      361 gcgcgcgttg atggggctcg cgacggagtt cgcccgcgag cggggcgccg ggcacctctg
      421 gctggaggtc accaacgtca acgcaccggc gatccacgcg taccggcgga tggggttcac
      481 cctctgcggc ctggacaccg ccctgtacga cggcaccgcc tcggacggcg agcaggcgct
      541 ctacatgagc atgccctgcc cctaatcagt actgacaata aaaagattct tgttttcaag
      601 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
      661 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
      721 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
      781 tactaacgcc gccatccagt gtcgaaaacg cggtgtgaaa taccgcacag atgcgtaagg
      841 agaaaatacc gcagcgtatt accttctgct ggattcaaac actcttctcc agtaaaa
//
