LOCUS       sequence-157444-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with 
            NLS, a Thosea asigna virus peptide (2A), and membrane-tethered 
            mCherry protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(139..846)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(414..433)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     683..701
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(853..906)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggttaatacg actcactata gggagtccgg atacgtaata cgactcacta tagttctaga
       61 tcaggagagc acacacttgc agctcatgca gccggggcca ctctcatcag gagggttcag
      121 cttagatctt cctcctccct tgtacagctc gtccatgccg ccggtggagt ggcggccctc
      181 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
      241 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
      301 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
      361 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
      421 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      481 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      541 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
      601 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
      661 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      721 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
      781 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      841 caccatagat ctgggcccgg gattttcctc cacgtccccg catgttagaa gacttcccct
      901 gccctctcct gatccgatat ctttttctgc actacgcagg gatatttcac cgcccatcca
      961 gggttttatt attccatcct gctcaagtaa taaag
//
