LOCUS       sequence-157697-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing CGRRF1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CGRRF1_WT_V5
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    541..552
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 atggctgcgg tgtttctggt aacgctttat gaatactcgc cgcttttcta catcgcggtg
       61 gtctttacct gcttcatcgt gaccaccggc ctggtattgg gatggtttgg ttgggatgtt
      121 ccagtaattc tgagaaattc agaagagacc cagttcagca caagagtttt caaaaagcaa
      181 atgagacaag tcaagaatcc ttttggctta gagatcacta atccatcttc agcttcaatt
      241 acaactggca taaccttgac aacagattgc cttgaagata gcctccttac atgctactgg
      301 gggtgcaatg ttcaaaaatt atatgaagct ctgcagaagc atgtttattg cttcagaata
      361 agcactcccc aagcattaga agatgctctg tatagtgaat atctctatca ggaacagtat
      421 tttattaaaa aggatagcaa agaagaaata tattgccagt taccaagaga tactaaaatt
      481 gaagactttg gtacagtacc cagatctcgc tatccattgg tagcgctatt gaccttagct
      541 gatgaggatg accgggaaat ttatgatatt atttccatgg tgtcagtgat tcatattcct
      601 gataggactt ataaactatc ctgcagaata ttgtatcaat atttactctt ggctcaaggt
      661 caatttcatg atcttaagca acttttcatg tctgcaaata ataatttcac tccctccaac
      721 aattcctctt cagaagaaaa aaacacagac agaagtttgt tggaaaaggt gggactctct
      781 gaaagtgaag ttgagccatc ggaagagaac agcaaggact gtgttgtttg ccagaatggg
      841 actgtgaact gggtactctt accatgcaga cacacatgcc tgtgtgatgg ctgtgtgaag
      901 tattttcagc agtgcccaat gtgcaggcag tttgttcagg aatcttttgc actttgcagt
      961 caaaaagagc aagataaaga caaaccgaag actctttga
//
