LOCUS       sequence-157776-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Biosensor for PtdIns4P.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Hammond GR, Machner MP, Balla T
  TITLE     A novel probe for phosphatidylinositol 4-phosphate reveals multiple 
            pools beyond the Golgi.
  JOURNAL   J Cell Biol. 2014 Apr 7.
  PUBMED    24711504
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2014 Apr 7."
COMMENT     Sequence Label: GFP-P4M-SidM
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..441)
                     /codon_start=1
                     /product="high-affinity phosphatidylinositol 4-phosphate 
                     binding module of SidM (DrrA) from Legionella pneumophila 
                     (Hammond et al., 2014)"
                     /label=P4M
                     /translation="TASTENFKNVKEKYQQMRGDALKTEILADFKDKLAEATDEQSLKQ
                     IVAELKSKDEYRILAKGQGLTTQLLGLKTSSVSSFEKMVEETRESIKSQERQTIKIK"
     primer_bind     complement(556..577)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat ccttatttta tcttaatggt ttgtctttct tgagacttaa tactttctct
      181 tgtttcctca accattttct caaatgaaga cactgaactt gtctttaacc ctaaaagctg
      241 ggttgttaac ccttgcccct tagccaaaat tctatattca tctttacttt tcaattcagc
      301 aacaatttgc ttaaggctct gttcatcggt agcttcagcc agtttatcct tgaaatcagc
      361 caggatttct gtttttaaag catcacctcg catttgctga tatttttctt taacattttt
      421 aaagttttcc gtgcttgccg tggatctgag tccggaagca ctaccaccgt cgactgcaga
      481 attcgaagct tgagctcgag atctgagtcc ggacttgtac agctcgtcca tgccgagagt
      541 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      601 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      661 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      721 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      781 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      841 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcg
//
