LOCUS       sequence-157889-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Force free control for Actin-cpstFRET-Actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Guo J, Wang Y, Sachs F, Meng F
  TITLE     Actin stress in cell reprogramming.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5252-61. doi: 
            10.1073/pnas.1411683111. Epub 2014 Nov 24.
  PUBMED    25422450
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1411683111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-9- 9"; volume: "111"; issue: "49"; pages: "E5252-61"
COMMENT     Sequence Label: cpstFRET-Actin
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..788
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKEFGGGGGVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKILHNIE"
     primer_bind     complement(315..336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(576..595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagagctggt ttagtgaacc gtcagatccg ctagccaggg acccggggta ccaatgggcg
       61 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      121 tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc aacgagaagc
      181 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      241 agctgtacaa ggaattcggc ggcggtggcg gggtgagcaa gggcgaggag ctgttcaccg
      301 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      361 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
      421 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
      481 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      541 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      601 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      661 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      721 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atcctccaca
      781 acatcgagga cggcggcaga tctatgggca gcgtgcagct cgccgaccac taccagcaga
      841 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      901 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      961 ccgcc
//
