LOCUS       sequence-157902-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: OPRM1-Tango
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..245)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     924..943
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cctctagact acccaccgta ctcgtcaatt ccaagggcat cggtaaacat ctgctcaaac
       61 tcgaagtcgg ccatatccag agcgccgtag ggggcggagt cgtggggggt aaatcccgga
      121 cccggggaat ccccgtcccc caacatgtcc agatcgaaat cgtctagcgc gtcggcatgc
      181 gccatcgcca cgtcctcgcc gtctaagtgg agctcgtccc ccaggctgac atcggtcggg
      241 ggggccgtcg acagtctgcg cgtgtgtccc gcggggagaa aggacaggcg cggagccgcc
      301 agccccgcct cttcgggggc gtcgtcgtcc gggagatcga gcaggccctc gatggtagac
      361 ccgtaattgt ttttcgtacg cgcgcggctg tacgcggacc cactttcaca tttaagttgt
      421 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
      481 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
      541 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
      601 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
      661 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
      721 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
      781 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
      841 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
      901 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccga
//
